CDK1, not ERK1/2 or ERK5, is required for mitotic phosphorylation of BIM sub(EL)

The pro-apoptotic BH3 only protein BIM sub(EL is phosphorylated by ERK1/2 and this targets it for proteasome-dependent degradation. A recent study has shown that ERK5, an ERK1/2-related MAPK, is activated during mitosis and phosphorylates BIM) sub(E)L to promote cell survival. Here we show that trea...

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Bibliographic Details
Published in:Cellular signalling 2012-01, Vol.24 (1), p.170-180
Main Authors: Gilley, Rebecca, Lochhead, Pamela A, Balmanno, Kathryn, Oxley, David, Clark, Jonathan, Cook, Simon J
Format: Article
Language:English
Subjects:
Control
Degradation
Fibroblasts
Inhibitors
Kinases
Mitosis
Phosphorylation
Survival
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Summary:The pro-apoptotic BH3 only protein BIM sub(EL is phosphorylated by ERK1/2 and this targets it for proteasome-dependent degradation. A recent study has shown that ERK5, an ERK1/2-related MAPK, is activated during mitosis and phosphorylates BIM) sub(E)L to promote cell survival. Here we show that treatment of cells with nocodazole or paclitaxel does cause phosphorylation of BIM sub(EL, which is independent of ERK1/2. However, this was not due to ERK5-catalysed phosphorylation, since it was not reversed by the MEK5 inhibitor BIX02189 and proceeded normally in ERK5-/- fibroblasts. Indeed, although ERK5 is phosphorylated at multiple sites in the C-terminal transactivation region during mitosis, these do not include the activation-loop and ERK5 kinase activity does not increase. Mitotic phosphorylation of BIM) sub(E)L occurred at proline-directed phospho-acceptor sites and was abolished by selective inhibition of CDK1. Furthermore, cyclin B1 was able to interact with BIM and cyclin B1/CDK1 complexes could phosphorylate BIM in vitro. Finally, we show that CDK1-dependent phosphorylation of BIM sub(EL drives its polyubiquitylation and proteasome-dependent degradation to protect cells during mitotic arrest. These results provide new insights into the regulation of BIM) sub(E)L and may be relevant to the therapeutic use of agents such as paclitaxel.
ISSN:0898-6568
DOI:10.1016/j.cellsig.2011.08.018