Biosynthesis of silver nanoparticles from Tribulus terrestris and its antimicrobial activity: A novel biological approach

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2012-08, Vol.96, p.69-74
• Main Authors: Gopinath, V., MubarakAli, D., Priyadarshini, S., Priyadharsshini, N. Meera, Thajuddin, N., Velusamy, P.
• Format: Article
• Language: English
• Subjects: Anti-Infective Agents - chemical synthesis; Anti-Infective Agents - chemistry; Anti-Infective Agents - pharmacology; Antibacterial; antibacterial properties; Bacillus subtilis; Bacillus subtilis - drug effects; Bacillus subtilis - growth & development; Bacteria; Bacteria - drug effects; Bacteria - growth & development; Biosynthesis; colloids; dried fruit; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - growth & development; Extraction plants; Fourier transform infrared spectroscopy; Fruit bodies; fruiting bodies; Fruits; Green chemistry; medicine; Metal Nanoparticles - chemistry; Metal Nanoparticles - ultrastructure; Microbial Sensitivity Tests; Microscopy, Atomic Force; Microscopy, Electron, Transmission; multiple drug resistance; Nanoparticles; nanosilver; Particle Size; phytopharmaceuticals; plant extracts; Plant Extracts - chemistry; Plants (organisms); Pseudomonas aeruginosa; Pseudomonas aeruginosa - drug effects; Pseudomonas aeruginosa - growth & development; Silver; Silver - chemistry; Silver nanoparticles; silver nitrate; Silver Nitrate - chemistry; Spectrophotometry; Spectroscopy, Fourier Transform Infrared; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus aureus - growth & development; Streptococcus; Streptococcus pyogenes - drug effects; Streptococcus pyogenes - growth & development; Synthesis; TEM; therapeutics; transmission electron microscopes; transmission electron microscopy; Tribulus - chemistry; Tribulus terrestris; Tribulus terrestris L; X-Ray Diffraction
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2012.03.023

[Display omitted] ► Synthesis of silver nanoparticles (Ag) by biological techniques with plant dried fruit body. ► The work emphasizes on the synthesis of silver nanoparticles with extracts of fruit body of the plant Tribulus terrestris. ► By this biological procedure, spherical shaped silver nanoparticle obtained similar to previous report. ► Apart from various microorganisms and biomolecules and plant based synthesis study, this may be the first report on the dried fruits based synthesis of Silver nanoparticles and stabilization. In the recent decades, increased development of green synthesis of nanoparticles is inevitable because of its incredible applications in all fields of science. There were numerous work have been produced based on the plant and its extract mediated synthesis of nanoparticles, in this present study to explore that the novel approaches for the biosynthesis of silver nanoparticles using plant fruit bodies. The plant, Tribulus terrestris L. fruit bodies are used in this study, where the dried fruit body extract was mixed with silver nitrate in order to synthesis of silver nanoparticles. The active phytochemicals present in the plant were responsible for the quick reduction of silver ion (Ag+) to metallic silver nanoparticles (Ag0). The reduced silver nanoparticles were characterized by Transmission Electron Microscope (TEM), Atomic Force Microscope (AFM), XRD, FTIR, UV–vis spectroscopy. The spherical shaped silver nanoparticles were observed and it was found to be 16–28nm range of sizes. The diffraction pattern also confirmed that the higher percentage of silver with fine particles size. The antibacterial property of synthesized nanoparticles was observed by Kirby–Bauer method with clinically isolated multi-drug resistant bacteria such as Streptococcus pyogens, Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis and Staphylococcus aureus. The plant materials mediated synthesis of silver nanoparticles have comparatively rapid and less expensive and wide application to antibacterial therapy in modern medicine.