Analysis by LC/ESI-MS of iophenoxic acid derivatives and evaluation as markers of oral baits to deliver pharmaceuticals to wildlife

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2010-07, Vol.878 (22), p.1997-2002
• Main Authors: Ballesteros, Cristina, Camarero, Pablo R., Cristòfol, Carles, Vicente, Joaquín, Gortazar, Christian, de la Fuente, José, Mateo, Rafael
• Format: Article
• Language: English
• Subjects: Administration, Oral; Adults; Analysis; Analytical, structural and metabolic biochemistry; Animals; Animals, Wild - blood; Biological and medical sciences; Biomarkers - analysis; Derivatives; Disease control; Dosing; Drug Delivery Systems; Female; Fundamental and applied biological sciences. Psychology; General pharmacology; Iopanoic Acid - administration & dosage; Iopanoic Acid - analogs & derivatives; Iopanoic Acid - analysis; Iophenoxic acid; Male; Markers; Medical sciences; Oral vaccination; Pharmaceuticals; Pharmacology. Drug treatments; Serums; Spectrometry, Mass, Electrospray Ionization - methods; Sus scrofa - blood; Tuberculosis; Veterinary Drugs - administration & dosage; Veterinary Drugs - analysis; Wildlife; Wildlife management
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2010.05.035

Iophenoxic acid and its derivatives (methyl, ethyl, and propyl) are organic chemicals used as markers in baiting campaigns to deliver vaccines, pharmaceuticals, contraceptives or poisons to wildlife. In this study we develop a method of detection of IPA derivatives by LC/ESI-MS (using butyl-IPA as internal standard) obtaining a limit of detection and quantification in wild boar ( Sus scrofa) serum of 0.037 μg/ml and 0.123 μg/ml, respectively. The average recovery of IPA derivatives was 88% at levels >0.2 μg/ml, with coefficients of variation