Nano-HPLC-inductively coupled plasma mass spectrometry for arsenic speciation

A nano-high performance liquid chromatography-inductively coupled plasma mass spectrometry (nano-HPLC-ICPMS) method is developed, using a demountable direct injection high efficiency nebulizer (d-DIHEN), to reduce sample and mobile phase consumption, minimize organic waste generation, decrease analy...

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Bibliographic Details
Published in:Journal of analytical atomic spectrometry 2007, Vol.22 (9), p.1199-1205
Main Authors: Brennan, Ryan G., O’Brien Murdock, Su-Ann E., Farmand, Maryam, Kahen, Kaveh, Samii, Salar, Gray, Jessica M., Montaser, Akbar
Format: Article
Language:English
Subjects:
Arsenic
Chromatography
Mass spectrometry
Nanocomposites
Nanomaterials
Nanostructure
Separation
Sodium
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Summary:A nano-high performance liquid chromatography-inductively coupled plasma mass spectrometry (nano-HPLC-ICPMS) method is developed, using a demountable direct injection high efficiency nebulizer (d-DIHEN), to reduce sample and mobile phase consumption, minimize organic waste generation, decrease analysis time, and enhance separation efficiency. A HPLC column (50 mm [times] 0.3 mm id), packed with 3.5 [small mu]m C material, is explored for chromatographic separation of five arsenic species naturally present in the environment or introduced as a pollutant: sodium (meta)arsenite [As()], arsenic acid [As()], dimethylarsenic acid (DMA), disodium methylarsenate (MA), and -arsanilic acid (-ASA). A fast chromatographic separation of five arsenic species is achieved in less than 12 min at a solution flow rate of 0.9 [small mu]L min using a 50 nL sample injection. The HPLC-ICPMS interface provides well defined flow injection profiles at various concentrations, giving a correlation coefficient of 0.999 for each individual arsenic species calibration curve. Precision values for peak height and area of five arsenic species range from 0.5 to 6.5% RSD and absolute detection limits are within 0.4 to 5.4 pg arsenic, which are comparable to previously reported data at higher solution uptake rates (20 [small mu]L min to 1 mL min) and larger sample injection volumes (20-100 [small mu]L).
ISSN:0267-9477
1364-5544
DOI:10.1039/b703257a