An integrated platform for in vitro single-site cell electroporation: Controlled delivery and electrodes functionalization

This work presents an improved platform for single-site electroporation and controlled transfectants delivery. The device consists of a gold microelectrode array (MEA) with integrated microfluidics and nanostructured titanium dioxide (ns-TiO2) functionalized electrodes for the improvement of cell ad...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2012-07, Vol.170, p.182-188
Main Authors: Odorizzi, L., Ress, C., Collini, C., Morganti, E., Lorenzelli, L., Coppedè, N., Alabi, A.B., Iannotta, S., Cazzanelli, E., Vidalino, L., Macchi, P.
Format: Article
Language:English
Subjects:
Arrays
cell adhesion
Cell electroporation
cell viability
Devices
Electrodes
Electroporation
Functionalization
gold
green fluorescent protein
humans
Lab-on-cell
Microelectrode array (MEA)
Microfluidics
Nanostructure
plasmids
Platforms
Titanium dioxide
uterine cervical neoplasms
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Summary:This work presents an improved platform for single-site electroporation and controlled transfectants delivery. The device consists of a gold microelectrode array (MEA) with integrated microfluidics and nanostructured titanium dioxide (ns-TiO2) functionalized electrodes for the improvement of cell adhesion. Human cervical cancer cells (HeLa) have been successfully cultivated on chip surface using traditional protocols. The system has been previously tested by electroporating HeLa cells with Lucifer Yellow (LY) and then, in order to validate the approach and cell viability, with plasmid for the enhanced expression of Green Fluorescence Protein (pEGFP-N1) delivered through microchannels.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2011.06.027