Multiplexed detection of cardiac biomarkers in serum with nanowire arrays using readout ASIC

• Published in: Biosensors & bioelectronics 2012-05, Vol.35 (1), p.218-223
• Main Authors: Zhang, Guo-Jun, Chai, Kevin Tshun Chuan, Luo, Henry Zhan Hong, Huang, Joon Min, Tay, Ignatius Guang Kai, Lim, Andy Eu-Jin, Je, Minkyu
• Format: Article
• Language: English
• Subjects: Antibodies, Immobilized; Application specific integrated circuit; Arrays; Biological and medical sciences; Biomarkers - blood; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biosensing Techniques - statistics & numerical data; Biosensors; Biotechnology; Cardiac biomarkers; Creatine; Creatine Kinase, MB Form - blood; Creatine Kinase, MM Form - blood; Equipment Design; Fundamental and applied biological sciences. Psychology; Heart Diseases - blood; Heart Diseases - diagnosis; Humans; Kinases; Limit of Detection; Methods. Procedures. Technologies; Multiplexed detection; Multiplexing; Myocardial Infarction - blood; Myocardial Infarction - diagnosis; Nanocomposites; Nanomaterials; Nanowires; Point-of-Care Systems; Serum; Serums; Silicon; Silicon nanowire array; Troponin T - blood; Various methods and equipments
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2012.02.052

▸ Early detection of cardiac biomarkers for diagnosis of heart attack is the key. ▸ A label-free detection system consists of silicon nanowire sensor array and ASIC. ▸ The system performs simultaneous-multiplexed detection of 3 cardiac biomarkers. ▸ 100fg/ml cTnT, CK-MM, and CK-MB in untreated serum was simultaneously detected. ▸ The detection system leads to miniaturization of POC device for diagnostic. Early detection of cardiac biomarkers for diagnosis of heart attack is the key to saving lives. Conventional method of detection like the enzyme-linked immunosorbent assay (ELISA) is time consuming and low in sensitivity. Here, we present a label-free detection system consisting of an array of silicon nanowire sensors and an interface readout application specific integrated circuit (ASIC). This system provides a rapid solution that is highly sensitive and is able to perform direct simultaneous-multiplexed detection of cardiac biomarkers in serum. Nanowire sensor arrays were demonstrated to have the required selectivity and sensitivity to perform multiplexed detection of 100fg/ml troponin T, creatine kinase MM, and creatine kinase MB in serum. A good correlation between measurements from a probe station and the readout ASIC was obtained. Our detection system is expected to address the existing limitations in cardiac health management that are currently imposed by the conventional testing platform, and opens up possibilities in the development of a miniaturized device for point-of-care diagnostic applications.