Effects of laser and LED radiation on mitochondrial respiration in experimental endotoxic shock

• Published in: Lasers in medical science 2013-05, Vol.28 (3), p.785-790
• Main Authors: Buravlev, E. A., Zhidkova, T. V., Vladimirov, Y. A., Osipov, A. N.
• Format: Article
• Language: English
• Subjects: Animals; Dentistry; Disease Models, Animal; Fluorescence; Lasers; Lasers, Gas - therapeutic use; Lasers, Solid-State - therapeutic use; Light; Light-emitting diodes; Lipopolysaccharides - toxicity; Low-Level Light Therapy; Male; Medicine; Medicine & Public Health; Membrane Potential, Mitochondrial - radiation effects; Membranes; Mitochondria; Mitochondria - metabolism; Mitochondria - radiation effects; Nitric oxide; Optical Devices; Optics; Original Article; Oxygen Consumption - radiation effects; Photonics; Phototherapy; Quantum Optics; Radiation; Rats; Respiration; Rodents; Sepsis; Shock, Septic - metabolism; Shock, Septic - radiotherapy; Shock, Septic - therapy; Therapy
• ISSN: 0268-8921; 1435-604X
• DOI: 10.1007/s10103-012-1155-7

Effects of low-level laser therapy (442 and 532 nm) and LED radiation (650 nm) on mitochondrial respiration in experimental endotoxic shock have been studied. A model of experimental endotoxic shock in rats was obtained by intraperitoneal injection of lipopolysaccharide B. It was found that low-level laser therapy and LED radiation dramatically affected the rate of mitochondrial respiration in third and fourth states both in LPS-treated animals and in control experiments. The maximal increase of the mitochondrial respiration rate (of about 40 %) in LPS-treated animals was observed when blue laser was applied at the dose of 6 J/cm 2 . Measurements of inner mitochondrial membrane surface potential with fluorescence probe JC-1 in LPS-treated rats showed in approximately 10 % decrease of potential in LPS-treated animals compared to control.