Determination of non-steroidal anti-inflammatory drugs residues in animal muscles by liquid chromatography–tandem mass spectrometry

• Published in: Analytica chimica acta 2010-07, Vol.672 (1), p.85-92
• Main Authors: Jedziniak, Piotr, Szprengier-Juszkiewicz, Teresa, Olejnik, Małgorzata, Żmudzki, Jan
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Animals; Anti-Inflammatory Agents, Non-Steroidal - analysis; Cattle; Chemistry; Chickens; Chromatographic methods and physical methods associated with chromatography; Chromatography; Chromatography, Liquid; Drug Residues - analysis; Drugs; Exact sciences and technology; Horses; Liquid chromatography–tandem mass spectrometry; Liquids; Mass spectrometry; Matrix effect; Muscle, Skeletal - chemistry; Muscles; Non-steroidal anti-inflammatory drugs; Other chromatographic methods; Oxyphenbutazone - analysis; Phenylbutazone - analysis; Residues; Spectrometric and optical methods; Swine; Tandem Mass Spectrometry
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2010.04.031

A confirmatory method for the determination of residues of nine non-steroidal anti-inflammatory drugs and one metabolite in animal muscles has been developed. After enzymatic hydrolysis samples were extracted with acetonitrile and cleaned up using alumina and C 18 SPE cartridges. Liquid chromatography–tandem mass spectrometry was used for the separation and determination of analytes. The method was validated in bovine muscles, according to the Commission Decision 2002/657/EC criteria. Applicability of the method in the analysis of swine, horse and chicken muscles was checked by precision and recovery experiment. The influence of matrix effect on the quantification of non-steroidal anti-inflammatory drugs residues was investigated. The method was used for the confirmation of phenylbutazone and oxyphenbutazone in horse muscle sample.