Bioconjugation of MOF-5 for molecular sensing

Zn-BDC (BDC = 1,4-benzenedicarboxylate acid) or more commonly known as metal organic framework (MOF-5) was synthesized via chemical stirring method and bioconjugated with anti-bovine serum albumin (anti-BSA) through pendent group. The pendent group of organic linker on surface of MOF-5 has been acti...

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Bibliographic Details
Published in:Journal of porous materials 2014-02, Vol.21 (1), p.99-104
Main Authors: Kumar, Pawan, Deep, Akash, Paul, A. K., Bharadwaj, L. M.
Format: Article
Language:English
Subjects:
Catalysis
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Fluorescence
Microscopy
Photoluminescence
Physical Chemistry
Porous materials
Quenching
Stability
Stirring
Surface chemistry
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Summary:Zn-BDC (BDC = 1,4-benzenedicarboxylate acid) or more commonly known as metal organic framework (MOF-5) was synthesized via chemical stirring method and bioconjugated with anti-bovine serum albumin (anti-BSA) through pendent group. The pendent group of organic linker on surface of MOF-5 has been activated via EDC (1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide) based simple organic reaction and then bioconjugation of anti-BSA was achieved. Bioconjugation of anti-BSA on to MOF-5 has been used to study the interaction with BSA based on their fluorescence properties. Fluorescent microscopy revealed successful interaction of anti-BSA with BSA onto MOF-5 surface. In addition, BSA conjugated MOF-5 showed no loss of enantioselectivity and structure stability. Photoluminescence quenching of the developed probe was demonstrated to observe the presence of BSA.
ISSN:1380-2224
1573-4854
DOI:10.1007/s10934-013-9752-9