Comparison of extraction phases for a two-phase culture of a recombinant E. coli producing retinoids

To prevent degradation of intracellular retinoids through in situ extraction from the cells, a two-phase culture system was performed. Several organic solvents, including n-alkanes, mineral oils and cosmetic raw materials, were applied as the extraction phase. Of the n-alkanes, n-decane had the high...

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Bibliographic Details
Published in:Biotechnology letters 2014-03, Vol.36 (3), p.497-505
Main Authors: Jang, Hui-Jeong, Ha, Bo-Kyung, Kim, Jin-Woong, Jung, Kyung-Hwa, Ahn, Jiyoon, Yoon, Sang-Hwal, Kim, Seon-Won
Format: Article
Language:English
Subjects:
alkanes
Applied Microbiology
Biochemistry
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Biotechnology - methods
Chemical compounds
Cosmetics
Culture
E coli
Escherichia coli
Escherichia coli - growth & development
Escherichia coli - metabolism
Extraction
Life Sciences
Microbiology
mineral oil
Mineral oils
Minerals
Organic solvents
Original Research Paper
Phases
Raw materials
Recombinant
retinoids
Retinoids - isolation & purification
Retinoids - metabolism
Solvent extraction processes
Solvents
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Summary:To prevent degradation of intracellular retinoids through in situ extraction from the cells, a two-phase culture system was performed. Several organic solvents, including n-alkanes, mineral oils and cosmetic raw materials, were applied as the extraction phase. Of the n-alkanes, n-decane had the highest retinoid production as 134 mg/l after 72 h. For mineral oil, light and heavy mineral oil gave retinoid productions of 158 and 174 mg/l after 96 h, respectively. Of other materials, isopropyl myristate gave the highest retinoid production of 181 mg/l. These results indicate that many types of oils can be applied for retinoid production, and optimization of the in situ extraction process will lead to further improve of economical production for the industrial purpose.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-013-1385-1