Detection and characterization of lactose-utilizing Lactococcus spp. in natural ecosystems

• Published in: Applied and Environmental Microbiology 1995-02, Vol.61 (2), p.788-792
• Main Authors: KLIJN, N, WEERKAMP, A. H, DE VOS, W. M
• Format: Article
• Language: English
• Subjects: adn; agricultural soils; aguas residuales; amplification chaine polymerase; Animals; Bacteria; Bacteriology; Base Sequence; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; bovin laitier; Cattle; cheese; Cheese - microbiology; cheesemaking; cultivos iniciadores; culture starter; cultured milk; dairy cattle; dairy industry; Dairy products; Dairying; dechet industriel; desechos industriales; dna; DNA Primers - genetics; DNA, Bacterial - genetics; eau usee; Ecosystem; Ecosystems; ensilado; ensilage produit; enzimas; enzyme; enzymes; exploitation agricole; explotaciones agrarias; fabricacion del queso; farms; fromage; Fundamental and applied biological sciences. Psychology; ganado de leche; gene; genes; industria lechera; industrial wastes; industrie laitiere; Isolation and description; lactococcus; Lactococcus - genetics; Lactococcus - isolation & purification; Lactococcus - metabolism; Lactococcus lactis; Lactose - metabolism; lactoserum; lait; lait fermente; leche; leche fermentada; milk; Mission oriented research; Molecular Sequence Data; nisin; nisina; nysine; peau; piel animal; Polymerase Chain Reaction; proteasas; protease; proteases; queso; reaccion de cadenas de polimerasa; Ribonucleic acid; ribosomas; ribosome; ribosomes; RNA; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; saliva; salive; Sequence Homology, Nucleic Acid; silage; skin; sol agricole; Species Specificity; starter cultures; suelos agricolas; suero de la leche; technologie fromagere; wastewater; whey
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/aem.61.2.788-792.1995

The presence of lactose-utilizing Lactococcus species in nondairy environments was studied by using identification methods based on PCR amplification and (sub)species-specific probes derived from 16S rRNA sequences. Environmental isolates from samples taken on cattle farms and in the waste flow of a cheese production plant were first identified to the genus level, using a Lactococcus genus-specific probe. Isolates which showed a positive signal with this probe were further identified to the (sub)species level. Lactococcus lactis isolates were also characterized at the phenotypic level for the ability to hydrolyze arginine, to ferment citrate, and to produce proteases and bacteriocins. With specific PCR amplifications, the presence of sequences related to citP, coding for citrate permease; prtP, coding for protease; and nisA or nisZ, the structural genes for production of nisin A or nisin Z, respectively, was verified. By these methods, it was possible to isolate lactococci from various environmental sources, such as soil, effluent water, and the skin of cattle. The strains of L. lactis isolated differed in a number of properties, such as the ability to hydrolyze arginine or the absence of citP-related sequences, from those found in industrial starter cultures. The results indicate that the majority of the industrially produced lactococci do not survive outside the dairy environment, although natural niches are available. However, from those niches strains with the potential to be developed into novel starter cultures may be isolated.