Mediatorless amperometric bienzyme glucose biosensor based on horseradish peroxidase and glucose oxidase cross-linked to multiwall carbon nanotubes

• Published in: Mikrochimica acta (1966) 2014-04, Vol.181 (5-6), p.535-541
• Main Authors: Xu, Shuxia, Qi, Honglan, Zhou, Shiyi, Zhang, Xinfeng, Zhang, Chengxiao
• Format: Article
• Language: English
• Subjects: Albumin; Analysis; Analytical Chemistry; Biosensors; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Crosslinked polymers; Crosslinking; Dextrose; Electrical measurements; Electrodes; Electron transport; Glucose; Microengineering; Multi wall carbon nanotubes; Nanochemistry; Nanotechnology; Nanotubes; Original Paper; Oxidases; Peroxidase; Sensors
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-014-1175-z

We report on a bienzyme-channeling sensor for sensing glucose without the aid of mediator. It was fabricated by cross-linking horseradish peroxidase (HRP) and glucose oxidase (GOx) on a glassy carbon electrode modified with multiwalled carbon nanotubes (MWNTs). The bienzyme was cross-linked with the MWNTs by glutaraldehyde and bovine serum albumin. The MWNTs were employed to accelerate the electron transfer between immobilized HRP and electrode. Glucose was sensed by amperometric reduction of enzymatically generated H 2 O 2 at an applied voltage of −50 mV (vs. Ag/AgCl). Factors influencing the preparation and performance of the bienzyme electrode were investigated in detail. The biosensor exhibited a fast and linear response to glucose in the concentration range from 0.4 to 15 mM, with a detection limit of 0.4 mM. The sensor exhibited good selectivity and durability, with a long-term relative standard deviation of