Simultaneous determination of dopamine and uric acid using layer-by-layer graphene and chitosan assembled multilayer films

• Published in: Talanta (Oxford) 2013-12, Vol.117, p.359-365
• Main Authors: Weng, Xuexiang, Cao, Qingxue, Liang, Lixin, Chen, Jianrong, You, Chunping, Ruan, Yongmin, Lin, Hongjun, Wu, Lanju
• Format: Article
• Language: English
• Subjects: Ascorbic Acid - chemistry; Chitosan; Chitosan - chemistry; detection limit; dielectric spectroscopy; Dopamine; Dopamine - analysis; Electrochemical impedance spectroscopy; Electrochemical Techniques; electrochemistry; Electrodes; glassy carbon electrode; Graphene; Graphite - chemistry; Hydrogen-Ion Concentration; Layer-by-layer; Limit of Detection; Multilayers; oxidation; Oxidation-Reduction; Reproducibility of Results; Scanning electron microscopy; Self assembly; Solutions; Uric acid; Uric Acid - analysis
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2013.09.023

Multilayer films containing graphene (Gr) and chitosan (CS) were prepared on glassy carbon electrodes with layer-by-layer (LBL) assembly technique. After being characterized with cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM), the electrochemical sensor based on the resulted films was developed to simultaneously determine dopamine (DA) and uric acid (UA). The LBL assembled electrode showed excellent electrocatalytic activity towards the oxidation of DA and UA. In addition, the self-assembly electrode possessed an excellent sensing performance for detection of DA and UA with a linear range from 0.1μM to 140µM and from 1.0µM to 125µM with the detection limit as low as 0.05µM and 0.1µM based on S/N=3, respectively. •Graphene and chitosan were prepared with layer-by-layer assembly technique.•The assembled electrode showed excellent electrocatalytic activity towards the oxidation of dopamine and uric acid.•Individual or simultaneous determination of dopamine and uric acid was realized.