Determination of 26 veterinary antibiotics residues in water matrices by lyophilization in combination with LC–MS/MS

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2014-02, Vol.949-950, p.79-86
• Main Authors: Hu, Feng-Yang, He, Li-Min, Yang, Jian-Wen, Bian, Kui, Wang, Zong-Nan, Yang, Hai-Cui, Liu, Ya-Hong
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - analysis; Antibiotics; Chromatography; Chromatography, Liquid - methods; Drug Stability; Environmental water; Freeze Drying - methods; Fresh Water - chemistry; Ionization; Limit of Detection; Liquid chromatography–tandem mass spectrometry; Lyophilization; Reproducibility of Results; Residues; Standard deviation; Tandem Mass Spectrometry - methods; Tetracyclines; Veterinary; Veterinary Drugs - analysis; Waste water; Waste Water - chemistry; Water Pollutants - analysis
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2014.01.008

•Determination of 26 veterinary antibiotics in environmental water.•Simple sample pretreatment by lyophilization technique.•A sensitive and reliable LC–MS/MS method is firstly developed. A sensitive, simple and reliable multi-residue method was developed for the determination of 26 widely used veterinary antibiotics including 6 macrolides, 2 pleuromutilins, 4 tetracyclines, 2 lincosamides, 6 fluoroquinolones and 6 sulfonamides in different water matrices using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Water samples were lyophilized to dryness. Target compounds were separated on Zorbax SB-Aq column (150mm×2.1mm i.d., 3.5μm) and determined by LC–MS/MS operating in positive electrospray ionization mode. Spiked at concentration levels of 0.02, 0.4 and 4μgL−1, recoveries of all target compounds were over 70% except sulfaquinoxaline (59.0% at 0.02μgL−1) with relative standard deviations below 20%. Limits of detection (LOD) and limits of quantification (LOQ) of 26 drugs ranged from 0.1 to 6.5ngL−1 and from 0.3 to 19ngL−1, respectively. The developed method was successfully applied to the analysis of 26 antibiotics residues in fish pond water, groundwater, biogas digester water, and lagoon wastewater samples collected from local pig farms.