Evaluation and quantification of spectral information in tissue by confocal microscopy

A confocal imaging and image processing scheme is introduced to visualize and evaluate the spatial distribution of spectral information in tissue. The image data are recorded using a confocal laser-scanning microscope equipped with a detection unit that provides high spectral resolution. The process...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biomedical optics 2012-10, Vol.17 (10), p.106011-106011
Main Authors: Maeder, Ulf, Marquardt, Kay, Beer, Sebastian, Bergmann, Thorsten, Schmidts, Thomas, Heverhagen, Johannes T, Zink, Klemens, Runkel, Frank, Fiebich, Martin
Format: Article
Language:English
Subjects:
Animals
Assessments
Confocal
Drug Delivery Systems
Emulsions - chemistry
Emulsions - metabolism
Image Processing, Computer-Assisted - methods
In vitro testing
Indicators
Microscopy, Confocal - methods
Models, Biological
Oxazines - chemistry
Oxazines - metabolism
Penetration
Pharmaceutical Preparations - administration & dosage
Pharmaceutical Preparations - metabolism
Reproducibility of Results
Skin - chemistry
Skin - metabolism
Skin Absorption - physiology
Spectra
Swine
Uptakes
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A confocal imaging and image processing scheme is introduced to visualize and evaluate the spatial distribution of spectral information in tissue. The image data are recorded using a confocal laser-scanning microscope equipped with a detection unit that provides high spectral resolution. The processing scheme is based on spectral data, is less error-prone than intensity-based visualization and evaluation methods, and provides quantitative information on the composition of the sample. The method is tested and validated in the context of the development of dermal drug delivery systems, introducing a quantitative uptake indicator to compare the performances of different delivery systems is introduced. A drug penetration study was performed in vitro. The results show that the method is able to detect, visualize and measure spectral information in tissue. In the penetration study, uptake efficiencies of different experiment setups could be discriminated and quantitatively described. The developed uptake indicator is a step towards a quantitative assessment and, in a more general view apart from pharmaceutical research, provides valuable information on tissue composition. It can potentially be used for clinical in vitro and in vivo applications.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.jbo.17.10.106011