Cell internalization of BODIPY-based fluorescent dyes bearing carbohydrate residues

Red-absorbing BODIPY species have been prepared and their photophysical properties have been studied. The high fluorescence quantum yield of such compounds allowed us to study their cell internalization by fluorescence microscopy, using concentration as low as nM. The results indicate that the new s...

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Bibliographic Details
Published in:Dyes and pigments 2014-11, Vol.110, p.67-71
Main Authors: Papalia, Teresa, Siracusano, Gabriel, Colao, Ivana, Barattucci, Anna, Aversa, Maria Chiara, Serroni, Scolastica, Zappalà, Gabriella, Campagna, Sebastiano, Sciortino, Maria Teresa, Puntoriero, Fausto, Bonaccorsi, Paola
Format: Article
Language:English
Subjects:
Bearing
BODIPY
Carbohydrates
Dyes
Endocytosis
Fluorescence
Fluorescence microscopy
Fluorescent dyes
Luminescence
Microscopy
Pigments
Red-absorber
Residues
Sugar-moieties
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Summary:Red-absorbing BODIPY species have been prepared and their photophysical properties have been studied. The high fluorescence quantum yield of such compounds allowed us to study their cell internalization by fluorescence microscopy, using concentration as low as nM. The results indicate that the new species are internalized within VERO cells and that the clathrin-dependent mechanism plays a crucial role in the cell internalization of the dyes. New red-absorbing and emitting carbohydrate-containing BODIPY species are internalized within VERO cells, exhibiting negligible cytotoxicity. The clathrin-dependent mechanism plays a crucial role in the cell internalization of the BODIPY dyes. [Display omitted] •New distyryl BODIPY derivatives have been prepared and well characterized.•The new BODIPY dyes emit in the red region with high quantum yield.•Their cell internalization was demonstrated by fluorescence microscopy.•The sugar moieties seem influence cell uptake pathway.
ISSN:0143-7208
1873-3743
DOI:10.1016/j.dyepig.2014.05.022