How does iron deficiency disrupt the electron flow in photosystem I of lettuce leaves?

The changes observed photosystem I activity of lettuce plants exposed to iron deficiency were investigated. Photooxidation/reduction kinetics of P700 monitored as ΔA820 in the presence and absence of electron transport inhibitors and acceptors demonstrated that deprivation in iron decreased the popu...

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Bibliographic Details
Published in:Journal of plant physiology 2013-11, Vol.170 (16), p.1400-1406
Main Authors: Msilini, Najoua, Essemine, Jemâa, Zaghdoudi, Maha, Harnois, Johanne, Lachaâl, Mokhtar, Ouerghi, Zeineb, Carpentier, Robert
Format: Article
Language:English
Subjects:
Absorbance changes
chlorophyll
Chlorophyll - metabolism
culture media
Cyclic electron flow
Deprivation
Diuron - metabolism
electron transfer
Electron Transport
Fe deficiency
Fluorescence
Fluorescence measurements
Inhibitors
Iron
Iron - metabolism
Iron and steel plants
Kinetics
Lactuca - metabolism
Leaves
lettuce
Lettuces
Light
nutrient deficiencies
Oxidation-Reduction
P700 oxidation/re-reduction kinetics
Paraquat - metabolism
Photooxidation
Photosynthesis
photosystem I
Photosystem I Protein Complex - metabolism
Plant Leaves - metabolism
reducing agents
Spectrometry, Fluorescence
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Summary:The changes observed photosystem I activity of lettuce plants exposed to iron deficiency were investigated. Photooxidation/reduction kinetics of P700 monitored as ΔA820 in the presence and absence of electron transport inhibitors and acceptors demonstrated that deprivation in iron decreased the population of active photo-oxidizable P700. In the complete absence of iron, the addition of plant inhibitors (DCMU and MV) could not recover the full PSI activity owing to the abolition of a part of P700 centers. In leaves with total iron deprivation (0μM Fe), only 15% of photo-oxidizable P700 remained. In addition, iron deficiency appeared to affect the pool size of NADP+ as shown by the decline in the magnitude of the first phase of the photooxidation kinetics of P700 by FR-light. Concomitantly, chlorophyll content gradually declined with the iron concentration added to culture medium. In addition, pronounced changes were found in chlorophyll fluorescence spectra. Also, the global fluorescence intensity was affected. The above changes led to an increased rate of cyclic electron transport around PSI mainly supported by stromal reductants.
ISSN:0176-1617
1618-1328
DOI:10.1016/j.jplph.2013.05.004