Nanotips for single-step preparation of DNA for qPCR analysis

A single-step concentration and elution method is developed for detection of DNA in buffer, saliva, and blood. A nanotip capturing DNA using an electric field and capillary action is directly dissolved in buffer for qPCR analysis. The concentration yield and the relative parameters are compared with...

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Bibliographic Details
Published in:Analyst (London) 2013-06, Vol.138 (11), p.3135-3138
Main Authors: Kalyanasundaram, Dinesh, Kim, Jong-Hoon, Fotouhi, Gareth, Lee, Hyun-Boo, Hiraiwa, Morgan, Oh, Kieseok, Lee, Kyong-Hoon, Chung, Jae-Hyun
Format: Article
Language:English
Subjects:
Analytic Sample Preparation Methods - methods
Bacteriophage lambda - genetics
Blood
Buffers
Carbon Compounds, Inorganic - chemistry
Deoxyribonucleic acid
DNA - analysis
DNA - blood
DNA - genetics
DNA - isolation & purification
DNA, Viral - analysis
DNA, Viral - genetics
Genome, Human - genetics
Humans
Nanocomposites
Nanomaterials
Nanostructure
Nanotechnology - methods
Nanowires - chemistry
Polymerase Chain Reaction - methods
Saliva
Saliva - chemistry
Silicon Compounds - chemistry
Time Factors
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Summary:A single-step concentration and elution method is developed for detection of DNA in buffer, saliva, and blood. A nanotip capturing DNA using an electric field and capillary action is directly dissolved in buffer for qPCR analysis. The concentration yield and the relative parameters are compared with those of a commercial kit.
ISSN:0003-2654
1364-5528
DOI:10.1039/c3an00170a