Preparation and potential in vivo anti-influenza virus activity of low molecular-weight [kappa]-carrageenans and their derivatives

Degradation of native [kappa]-carrageenan was performed using acid hydrolysis aided with microwave heating. Combined with nonofiltration membrane (cut-off molecular weight 250 Da) separation, 1. 400 Da - 50 kDa low-molecular-weight (LMW) [kappa]-carrageenans were obtained. Narrow molecular weight di...

Full description

Saved in:
Bibliographic Details
Published in:Journal of applied polymer science 2013-02, Vol.127 (3), p.2110-2115
Main Authors: Tang, Fengxiang, Chen, Fang, Li, Feng
Format: Article
Language:English
Subjects:
Acetylation
Biomedical materials
Carrageenan
Derivatives
In vivo testing
In vivo tests
Materials science
Polymers
Sulfation
Surgical implants
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Degradation of native [kappa]-carrageenan was performed using acid hydrolysis aided with microwave heating. Combined with nonofiltration membrane (cut-off molecular weight 250 Da) separation, 1. 400 Da - 50 kDa low-molecular-weight (LMW) [kappa]-carrageenans were obtained. Narrow molecular weight distribution of LMW [kappa]-carrageenans could be prepared under pH 2.18 during the microwave power range investigated. The in vivo anti-influenza virus (IV) activity of three kinds of LMW [kappa]-carrageenans (3, 5, and 10 kDa), their acetylated derivatives (acetylation degree of 1.5), as well as an acetylated and sulfated derivative of 3 kDa carrageenan (acetylation degree of 1.0 and sulfation degree of 2.4), were investigated using FM1-induced pulmonary oedema model. These LMW [kappa]-carrageenans showed significant inhibition against FM1-induced pulmonary oedema as compared with the virus control, although their activities were inferior to that of positive control, Rabivirin. Introduction of acetyl groups greatly increased their anti-IV activity. The acetylated 3-kDa [kappa]-carrageenan exhibited comparative activity with Rabivirin at both doses of 6 and 30 2. mg/kg.d, and the acetylated and sulfated derivative of 3 kDa carrageenan displayed higher activity than Rabivirin at the dose of 30 mg/kg.d. These results disclosed that 3 kDa [kappa]-carrageenan with proper acetylation degree and sulfation degree was a potential candidate against influenza virus. [copy 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
ISSN:0021-8995
1097-4628
DOI:10.1002/app.37502