Biodegradation of 2,4-dichlorophenol by shiitake mushroom (Lentinula edodes) using vanillin as an activator

The white-rot shiitake mushroom, Lentinula edodes, was used to degrade an environmentally hazardous compound, 2,4-dichlorophenol (DCP), using vanillin as an activator. Vanillin increased the mycelial growth from 74 to 118 mg/150 ml culture and accelerated laccase and Mn-peroxidase production from th...

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Bibliographic Details
Published in:Biotechnology letters 2013-07, Vol.35 (7), p.1079-1083
Main Authors: Tsujiyama, S, Muraoka, T, Takada, N
Format: Article
Language:English
Subjects:
2,4-dichlorophenol
Applied Microbiology
Benzaldehydes - metabolism
Biochemistry
Biodegradation
Biomedical and Life Sciences
Biotechnology
Biotransformation
Chlorophenols - metabolism
Culture
Degradation
Dimers
Environmental degradation
Environmental Pollutants - metabolism
Enzymes
Gas Chromatography-Mass Spectrometry
Hazardous
Laccase
Laccase - metabolism
Lentinula edodes
Life Sciences
manganese peroxidase
Microbiology
Mushrooms
Mycelium - genetics
Mycelium - growth & development
Original Research Paper
Peroxidases - metabolism
phenol
Phenols
Shiitake Mushrooms - drug effects
Shiitake Mushrooms - enzymology
Shiitake Mushrooms - growth & development
Shiitake Mushrooms - metabolism
toxicity
Vanillin
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Summary:The white-rot shiitake mushroom, Lentinula edodes, was used to degrade an environmentally hazardous compound, 2,4-dichlorophenol (DCP), using vanillin as an activator. Vanillin increased the mycelial growth from 74 to 118 mg/150 ml culture and accelerated laccase and Mn-peroxidase production from the maximum on days 24–28 without vanillin to days 10–14. It eliminated 92 % of 100 mM DCP with 50 mg vanillin/l compared with only 15 % without vanillin. GC–MS revealed that a diaryl ether dimer of DCP was formed in the culture without vanillin, whereas dimer formation was diminished with vanillin addition. This indicates that vanillin enhances the degradation of DCP and disrupts the formation of the toxic dimer. Therefore, lignin-derived phenol such as vanillin can be used as natural and eco-friendly activators to control white-rot mushrooms, thereby facilitating the effective degradation of environmentally hazardous compounds.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-013-1179-5