Recombinant l-phenylalanine ammonia lyase from Rhodosporidium toruloides as a potential anticancer agent

The recombinant producer strain expressing Rhodosporidium toruloides l‐phenylalanine ammonia lyase (PAL) has been obtained, and a purification procedure of PAL has been developed. The purified enzyme, PAL, has the following biochemical and catalytic characteristics: Km for l‐Phe of 0.49 mM, pH optim...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology and applied biochemistry 2013-05, Vol.60 (3), p.316-322
Main Authors: Babich, Olga O., Pokrovsky, Vadim S., Anisimova, Natalia Yu, Sokolov, Nikolai N., Prosekov, Alexander Yu
Format: Article
Language:English
Subjects:
Ammonia
Animals
anticancer activity
anticancer activity, cytotoxic enzyme, recombinant enzyme
Antineoplastic Agents - pharmacology
Biochemistry
Biotechnology
Cell Line, Tumor
cytotoxic enzyme
Escherichia coli
Female
Humans
Hydrogen-Ion Concentration
l-phenylalanine ammonia lyase
Life span
MCF-7 Cells
Mice
Optimization
Phenylalanine Ammonia-Lyase - genetics
Phenylalanine Ammonia-Lyase - pharmacology
Purification
Recombinant
recombinant enzyme
Recombinant Proteins - genetics
Recombinant Proteins - pharmacology
Rhodosporidium toruloides
Temperature
Yeasts - genetics
Yeasts - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The recombinant producer strain expressing Rhodosporidium toruloides l‐phenylalanine ammonia lyase (PAL) has been obtained, and a purification procedure of PAL has been developed. The purified enzyme, PAL, has the following biochemical and catalytic characteristics: Km for l‐Phe of 0.49 mM, pH optimum at 8.5, and temperature optimum at 50°C. PAL exhibited a significant cytotoxic effect toward the following cell lines: MCF7 (IC50 = 1.97 U/mL), DU145 (IC50 = 7.3 U/mL), which are comparable with E. coli l‐asparaginase type‐II cytotoxicity in vitro. Administration of PAL (200–400 U/kg) to L5178y‐bearing mice for five times (a total dose of 1000–2000 U/kg) was well tolerated and showed the increase of life span (ILS) = 12–16%, P < 0.05. Data obtained suggest that PAL from R. toruloides has a potential for cancer treatment.
ISSN:0885-4513
1470-8744
DOI:10.1002/bab.1089