Antioxidant activities of chicken liver hydrolysates by pepsin treatment

This study was divided into two parts: (i) an optimal hydrolysing procedure of chicken liver hydrolysates (CLHs) and (ii) the in vivo antioxidant properties of CLHs via a D‐galactose‐induced mouse model. A pepsin‐to‐raw chicken liver mass ratio (1:400, w:w) and 2‐h hydrolysing period were chosen to...

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Bibliographic Details
Published in:International journal of food science & technology 2014-07, Vol.49 (7), p.1654-1662
Main Authors: Chou, Chung‐Hsi, Wang, Sheng‐Yao, Lin, Yi‐Tsen, Chen, Yi‐Chen
Format: Article
Language:English
Subjects:
Amino acid profile
animal models
antioxidant activity
Antioxidants
arsenic
aspartic acid
Biomedical materials
body weight
cadmium
catalase
chicken liver hydrolysate
Chickens
Food science
glutamic acid
Glutathione
glutathione peroxidase
hydrolysates
in vitro and in vivo antioxidant effects
In vivo testing
In vivo tests
Liver
manganese
manufacturing
mercury
mice
mineral profile
pepsin
Poultry
selenium
superoxide dismutase
Surgical implants
tin
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Summary:This study was divided into two parts: (i) an optimal hydrolysing procedure of chicken liver hydrolysates (CLHs) and (ii) the in vivo antioxidant properties of CLHs via a D‐galactose‐induced mouse model. A pepsin‐to‐raw chicken liver mass ratio (1:400, w:w) and 2‐h hydrolysing period were chosen to manufacture CLHs based on yield, peptide level and antioxidant effect. Molecular masses of CLHs were lower than 10 kDa. CLH was rich in aspartic acid and glutamic acid, and also contained both manganese and selenium, which are essential cofactors of superoxide dismutase and glutathione peroxidase, respectively. The contents of cadmium, mercury, tin, and arsenic in CLHs were very low and even no detectible. Regarding the in vivo antioxidant activity of CLHs, a dosage of 1.2 g D‐galactose kg⁻¹ body weight increased (P < 0.05) 2‐thiobarbituric acid reactive substances values and decreased (P < 0.05) glutathione and Trolox equivalent antioxidant capacity values, as well as superoxide dismutase, catalase, and glutathione peroxidase activities in serum and organs of mice. However, the in vivo antioxidant capacities were improved (P < 0.05) by supplementing CLHs.
ISSN:0950-5423
1365-2621
DOI:10.1111/ijfs.12471