Improved propionic acid production from glycerol with metabolically engineered Propionibacterium jensenii by integrating fed-batch culture with a pH-shift control strategy

•A two-stage pH control strategy was proposed for PA production by engineered P. jensenii.•PA titer was increased from 10.54g/L of control (pH uncontrolled) to 21.43g/L by pH-shift control.•Influence of different fed-batch culture modes on PA production was investigated.•The integration of fed-batch...

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Bibliographic Details
Published in:Bioresource technology 2014-01, Vol.152, p.519-525
Main Authors: Zhuge, Xin, Liu, Long, Shin, Hyun-dong, Li, Jianghua, Du, Guocheng, Chen, Jian
Format: Article
Language:English
Subjects:
Batch Cell Culture Techniques - methods
Biological and medical sciences
Culture
Fermentation
Fundamental and applied biological sciences. Psychology
Glycerol
Glycerol - metabolism
Glycerols
Hydrogen-Ion Concentration
Kinetics
Metabolic Engineering
Propionates - metabolism
Propionibacterium - growth & development
Propionibacterium - metabolism
Propionibacterium jensenii
Propionic acid
Strategy
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Summary:•A two-stage pH control strategy was proposed for PA production by engineered P. jensenii.•PA titer was increased from 10.54g/L of control (pH uncontrolled) to 21.43g/L by pH-shift control.•Influence of different fed-batch culture modes on PA production was investigated.•The integration of fed-batch culture with two-stage pH control further increased PA production. Propionic acid (PA) production with metabolically engineered Propionibacterium jensenii (pZGX04-gldA) was improved by integrating fed-batch culture with a two-stage pH control strategy in a 3-L fermenter. The following two-stage pH control strategy was used: the pH was controlled at 5.9 for 0–36h and shifted to 6.5 after 36h. The PA titer was increased to 21.43g/L. On the basis of pH control, the influence of fed-batch culture on PA production was further investigated and the maximum PA production (34.62g/L) was obtained when glycerol was fed at a constant rate of 3.33mL/h from 60 to 132h with an initial glycerol concentration of 25g/L. Crude glycerol was then used to produce PA using the optimized strategies, and maximal PA production reached 37.26g/L. The strategies may be useful for the production of PA by other propionibacteria species.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2013.11.063