Anthocyanin identification and composition of wild Vitis spp. accessions by using LCaMS and LCaNMR

The composition and concentration of anthocyanins of grape berry skins were analyzed in order to assess phenotypic variation between four grape wine varieties belonging to 4 different species: Vitis vinifera, Vitis amurensis, Vitis cinerea and Vitis X champinii. High-performance liquid chromatograph...

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Bibliographic Details
Published in:Analytica chimica acta 2012-06, Vol.732, p.145-152
Main Authors: Acevedo De la Cruz, Alexander, Hilbert, Ghislaine, RiviA"re, Celine, Mengin, Virginie, Ollat, Nathalie, Bordenave, Louis, Decroocq, Stephane, Delaunay, Jean-Claude, Delrot, Serge, Merillon, Jean-Michel, Monti, Jean-Pierre, GomA"s, Eric, Richard, Tristan
Format: Article
Language:English
Subjects:
Anthocyanins
Concentration (composition)
Derivatives
Grapes
Isomers
Mass spectrometry
Mathematical analysis
NMR spectroscopy
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Summary:The composition and concentration of anthocyanins of grape berry skins were analyzed in order to assess phenotypic variation between four grape wine varieties belonging to 4 different species: Vitis vinifera, Vitis amurensis, Vitis cinerea and Vitis X champinii. High-performance liquid chromatography coupled to mass spectrometry (LCaMS) and NMR spectroscopy (LCaNMR) were used to separate and identify the structure of anthocyanins present in these species. Combination of LCaMS and LCaNMR data resulted in the identification of 33 anthocyanins. In particular, newly reported cis isomers of p-coumaric-derivatives were identified (petunidin-, peonidin- and malvidin-3-(6-p-coumaroyl)-5-diglucoside). In V. cinerea and V. vinifera, anthocyanins were monoglucoside derivatives whereas in V. amurensis and V. X champinii, both mono- and diglucoside derivatives were identified. Malvidin-, delphinidin- and petunidin-derivatives were, respectively, the most abundant components in V. cinerea and V. vinifera, V. amurensis and V. X champinii.
ISSN:0003-2670
DOI:10.1016/j.aca.2011.11.060