Lactobacillus brevis OPK‐3 isolated from kimchi inhibits adipogenesis and exerts anti‐inflammation in 3T3‐L1 adipocyte

• Published in: Journal of the science of food and agriculture 2014-09, Vol.94 (12), p.2514-2520
• Main Authors: Park, Jeong‐Eun, Oh, Suk‐Heung, Cha, Youn‐Soo
• Format: Article
• Language: English
• Subjects: 3T3-L1 Cells; Adipocytes - metabolism; Adipogenesis - drug effects; Adipogenesis - genetics; adipogenesis transcription factors genes; adiponectin; Adiponectin - genetics; Adiponectin - metabolism; Animals; Anti-Inflammatory Agents - pharmacology; Bacteria; cytokine genes; Cytokines; Cytokines - genetics; Cytokines - metabolism; Down-Regulation; Ethnic foods; fatty acid-binding proteins; Fermentation; Foods; Gene expression; gene expression regulation; Genes; Inflammation - genetics; Inflammation - metabolism; Kimchi; lactic acid bacteria; Lactobacillus; Lactobacillus brevis; Lactobacillus brevis - isolation & purification; Lactobacillus brevis OPK-3; leptin; Lipid Metabolism - genetics; Lipids; lipogenesis; lipoprotein lipase; liver; messenger RNA; Mice; Microorganisms; Probiotics; Receptors; RNA, Messenger - metabolism; transcription factors; Transcription Factors - metabolism; Triglycerides - metabolism; tumor necrosis factor-alpha; Vegetables - microbiology
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.6588

BACKGROUND: Kimchi is a traditional fermented food in Korea that contains various unique microorganisms. Diverse bacteria are involved in the process of Kimchi fermentation and the healthful advantages; one of the major species is Lactobacillus. We investigated whether lactic acid bacteria isolated from Kimchi (KLAB) are capable of reducing intracellular lipid accumulation by downregulating the expression of adipogenesis and lipogenesis promoting genes in differentiating 3T3‐L1 cells. RESULTS: KLAB (Lactobacillus brevis OPK‐3) mediated dose‐dependent inhibition of adipocyte differentiation, intracellular triglyceride accumulation and glycerol‐3‐phosphate dehydrogenase (GPDH) activity. The expression of transcription factors such as peroxisome proliferator‐activated receptor γ and CCAAT/enhancer‐binding protein α involved in adipogenesis was markedly decreased by the KLAB treatment. Terminal adipogenic marker, e.g. adipocyte fatty acid binding protein (aP2), lipoprotein lipase, liver X receptor α, leptin and GPDH were significantly downregulated by KLAB treatment compared to untreated control. Moreover, cytokine genes, such as tumor necrosis factor‐α and interleukin‐6 mRNA expressions level were also decreased, whereas adiponectin mRNA level was upregulated by KLAB. CONCLUSION: These results suggest that the KLAB inhibits lipid accumulation in the differentiating adipocyte through downregulating the expression of adipogenic transcription factors and other specific genes involved in lipid metabolism. © 2014 Society of Chemical Industry