Two-dimensional subpicosecond time-resolved fluorescence anisotropy: Optical Kerr-gating with the excitation of alternating polarizations of light

We have developed a subpicosecond time-resolved fluorescence anisotropy (TRFA) that newly implements a photoelastic modulator to alternate the polarizations of an excitation laser light. The setup facilitates virtually simultaneous detection of the parallel I∥ and the perpendicular I⊥ emission from...

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Bibliographic Details
Published in:Optics communications 2014-03, Vol.315, p.324-328
Main Authors: Fujiwara, Takashige, Romano, Natalie C., Lim, Edward C.
Format: Article
Language:English
Subjects:
Anisotropy
Charge coupled devices
Excitation
Fluorescence
Fluorescence anisotropy
Methyl alcohol
Optical Kerr gating
Photoelastic modulator
Polarization
Spectra
Two dimensional
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Summary:We have developed a subpicosecond time-resolved fluorescence anisotropy (TRFA) that newly implements a photoelastic modulator to alternate the polarizations of an excitation laser light. The setup facilitates virtually simultaneous detection of the parallel I∥ and the perpendicular I⊥ emission from a photoexcited molecule of interest by means of an ultra-short (optical Kerr-gating) shutter and a spectrograph coupled with a charge-coupled device. From a set of I∥(λ,t) and I⊥(λ,t) that comprise 2-D (two-dimensional) information on both a full range of spectra and subpicosecond time-resolved fluorescence decays, the 2-D TRFA, R(λ,t), is directly given with better accuracy. To claim the merit of the technique we carried out a test for 2-D TRFA of Coumarin 153 in methanol.
ISSN:0030-4018
1873-0310
DOI:10.1016/j.optcom.2013.11.039