Potentiation of non-halocarbon oxidants on halocarbon-induced oxidative damage to rat red blood cells

We have studied individual and mixture toxicities of halocarbons and non-halocarbons in rat red blood cells (rRBC). Hemolysis and release of TBARS, hemoglobin (Hb), and methemoglobin (MetHb) as well as release of glutathione peroxidase and catalase were determined. Tetrahalocarbons were more damagin...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1992-11, Vol.40 (11), p.2192-2197
Main Authors: Sano, Mitsuaki, Hu, Miao Lin, Tappel, A. L
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chemical and industrial products toxicology. Toxic occupational diseases
eritrocitos
erythrocyte
erythrocytes
halogenated hydrocarbons
hidrocarburos halogenados
hydrocarbure halogene
ingestion toxicity
Medical sciences
oxidantes
oxidants
oxydant
rat
rata
rats
Solvents
toxicidad por ingestion
toxicite par ingestion
Toxicology
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Summary:We have studied individual and mixture toxicities of halocarbons and non-halocarbons in rat red blood cells (rRBC). Hemolysis and release of TBARS, hemoglobin (Hb), and methemoglobin (MetHb) as well as release of glutathione peroxidase and catalase were determined. Tetrahalocarbons were more damaging than dihalocarbons, and bromoethanes were more damaging to rRBC than chloroethanes. tert-Butyl hydroperoxide (BHP) was most damaging to rRBC by every measurement, while H2O2 had no effect. 2,2-Azobis(2-amidinopropane) hydrochloride (AAPH) increased MetHb in the medium but decreased hemolysis. BHP strongly potentiated the damage of rRBC induced by BrCCl(3) (0.5 millimoles), including a 100% hemolysis within 2 h at 37 degrees C. BHP potentiated the release of MetHb from rRBC incubated with C2H2Br4 or C2H2Cl4 but not with C2H4Br2 or C2H4Cl2. The potentiation of BHP on the release of MetHb was selective as BHP did not affect lipid peroxidation or hemolysis. AAPH significantly inhibited hemolysis induced by C2H2Br4 and C2H2Cl4 and release of Hb induced by C2H2Cl4. AAPH did not affect lipid peroxidation or MetHb release induced by these halocarbons. Thus, the mixture of a halocarbon and a non-halocarbon led to mostly potentiating toxicity, although antagonistic effects could occur depending on the type of oxidant mixture used. Moreover, lipid peroxidation and Hb oxidation were selectively affected by the oxidant mixture.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf00023a029