Expression of the pCloDF13 encoded bacteriocin release protein or its stable signal peptide causes early effects on protein biosynthesis and Mg super(2+) transport

The effect of the pCloDF13 encoded bacteriocin release protein (BRP) on Escherichia coli cell lethality was studied. Induction of the BRP resulted in a strong inhibition of the incorporation of radioactive labeled amino acids and affected the transport of Mg super(2+) ions. Similar effects were obta...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek 1995-01, Vol.67 (3), p.255-260
Main Authors: Stegehuis, F, Van der Wal, FJ, Luirink, J, Oudega, B
Format: Article
Language:English
Subjects:
Escherichia coli
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Summary:The effect of the pCloDF13 encoded bacteriocin release protein (BRP) on Escherichia coli cell lethality was studied. Induction of the BRP resulted in a strong inhibition of the incorporation of radioactive labeled amino acids and affected the transport of Mg super(2+) ions. Similar effects were obtained when the BRP stable signal peptide was expressed as a separate entity. Kinetic studies revealed that these effects occurred prior to quasi-lysis and release of cloacin DF13. The results indicated that the BRP induced cell lethality is caused by early effects on protein synthesis and Mg super(2+) transport, due to the accumulation of stable BRP signal peptides in the cytoplasmic membrane.
ISSN:0003-6072