Comparative analysis of genetic variability among Borrelia burgdorferi isolates from Europe and the United States by restriction enzyme analysis, gene restriction fragment length polymorphism, and pulsed-field gel electrophoresis

The genomes of 62 North American and European Borrelia burgdorferi isolates were examined by restriction endonuclease analysis (REA), gene probe restriction fragment length polymorphism, and pulsed-field gel electrophoresis (PFGE). Hybridization of restriction fragments with the immunologically rele...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 1993-12, Vol.31 (12), p.3115-3122
Main Authors: Zingg, B.C, Anderson, J.F, Johnson, R.C, LeFebvre, R.B
Format: Article
Language:English
Subjects:
Animals
Antigens, Bacterial - genetics
Bacteriology
Biological and medical sciences
BORRELIA BURGDORFERI
Borrelia burgdorferi Group - genetics
Borrelia burgdorferi Group - immunology
Borrelia burgdorferi Group - isolation & purification
DNA Restriction Enzymes
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
Electrophoresis, Gel, Pulsed-Field
Epidemiology
ESTADOS UNIDOS DE AMERICA
ETATS UNIS
EUROPA
EUROPE
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genetic Variation
Humans
Microbiology
Polymorphism, Restriction Fragment Length
United States
VARIACION GENETICA
VARIATION GENETIQUE
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Summary:The genomes of 62 North American and European Borrelia burgdorferi isolates were examined by restriction endonuclease analysis (REA), gene probe restriction fragment length polymorphism, and pulsed-field gel electrophoresis (PFGE). Hybridization of restriction fragments with the immunologically relevant 83-kDa antigen gene revealed polymorphisms and divided the isolates into three major groups. Group I included all but two of the American isolates and some of the European isolates. One of two Californian isolates (DN 127) and one Ixodes dammini isolate from New York (strain 25015), previously described as atypical, were distinct from the isolates in the three groups. Plasmid profile analysis and REA, the method with the highest level of discrimination, revealed extensive heterogeneity among isolates of the same major group. Our study demonstrates the usefulness of the 83-kDa antigen gene probe for dividing the isolates into major genogroups, whereas REA and plasmid profile analysis allow for a distinction of individual strains within these groups
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.31.12.3115-3122.1993