Multi-residue Determination of Polar Veterinary Drugs in Livestock and Fishery Products by Liquid Chromatography/Tandem Mass Spectrometry

Residues of 37 polar veterinary drugs belonging to six families (quinolones, tetracyclines, macrolides, lincosamides, sulfonamides, and others) in livestock and fishery products were determined using a validated LC-MS/MS method. There were two key points in sample preparation. First, extraction was...

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Bibliographic Details
Published in:Journal of AOAC International 2015-01, Vol.98 (1), p.230-247
Main Authors: Kanda, Maki, Nakajima, Takayuki, Hayashi, Hiroshi, Hashimoto, Tsuneo, Kanai, Setsuko, Nagano, Chieko, Matsushima, Yoko, Tateishi, Yukinari, Yoshikawa, Soichi, Tsuruoka, Yumi, Sasamoto, Takeo, Takano, Ichiro
Format: Article
Language:English
Subjects:
Animals
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Chromatography, Liquid - methods
Chromatography, Liquid - veterinary
Drug Residues - chemistry
Drugs
Eggs - analysis
Fish industry
Fish Products - analysis
Fisheries
Formic acid
Honey - analysis
Liquid chromatography
Livestock
Livestock industry
Mass spectrometry
Milk - chemistry
Muscle, Skeletal - chemistry
Nitriles
Organic acids
Product introduction
Tandem Mass Spectrometry - methods
Tandem Mass Spectrometry - veterinary
Veterinary Drugs - chemistry
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Summary:Residues of 37 polar veterinary drugs belonging to six families (quinolones, tetracyclines, macrolides, lincosamides, sulfonamides, and others) in livestock and fishery products were determined using a validated LC-MS/MS method. There were two key points in sample preparation. First, extraction was performed with two solutions of different polarity. Highly polar compounds were initially extracted with Na2EDTA-McIlvaine's buffer (pH 7.0). Medium polar compounds were then extracted from the same samples with acetonitrile containing 0.1% formic acid. Secondly, cleanup was performed using a single SPE polymer cartridge. The first extracted solution was applied to the cartridge. Highly polar compounds were retained on the cartridge. Then, the second extracted solution was applied to the same cartridge. Both highly and medium polar compounds were eluted from the cartridge. This method satisfied the guideline criteria for 37 out of 37 drugs in swine muscle, chicken muscle, bovine muscle, prawn, salmon trout, red sea bream, milk, and honey; 35 out of 37 in egg; and 34 out of 37 in flounder. The LOQ ranged from 0.1 to 5 μg/kg. Residues were detected in 24 out of 110 samples and analyzed using the validated method.
ISSN:1060-3271
1944-7922
DOI:10.5740/jaoacint.13-272