Transformation of Bacillus brevis 47 by electroporation

Bacillus brevis 47 was successfully and reproducibly transformed with pUB110 plasmid DNA by electroporation with an efficiency of 10 super(4) transformants per mu g of DNA. This represents a 10-fold improvement over the chemical transformation method previously used for this organism.

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Bibliographic Details
Published in:Biotechnology techniques 1993, Vol.7 (1), p.47-50
Main Authors: WHITNEY, G. D, DAUGULI, A. J, WHITE, B. N
Format: Article
Language:English
Subjects:
Bacillus brevis
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Miscellaneous
Synthetic digonucleotides and genes. Sequencing
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Description
Summary:Bacillus brevis 47 was successfully and reproducibly transformed with pUB110 plasmid DNA by electroporation with an efficiency of 10 super(4) transformants per mu g of DNA. This represents a 10-fold improvement over the chemical transformation method previously used for this organism.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF00151089