Determination of the Heroin Metabolite 6-Monoacetylmorphine in Urine by High-Performance Liquid Chromatography with Electrochemical Detection

A procedure has been developed for detection and quantitation of 6-monoecetylmorphine (6-MAM), the specific metebolite of heroin in human urine. 6-MAM was isolated by solid-phase extraction, and the extracts were analyzed by high-performance liquid chromatography with electrochemical detection in th...

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Bibliographic Details
Published in:Journal of analytical toxicology 1993-01, Vol.17 (1), p.48-50
Main Authors: Hanisch, W., Meyer, L.V.
Format: Article
Language:English
Subjects:
Heroin
High-performance liquid chromatography
Metabolites
Quantitation
Urine
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Summary:A procedure has been developed for detection and quantitation of 6-monoecetylmorphine (6-MAM), the specific metebolite of heroin in human urine. 6-MAM was isolated by solid-phase extraction, and the extracts were analyzed by high-performance liquid chromatography with electrochemical detection in the oxidizing mode. Good linearity was obtained in the concentration range 10–250 ng/mL. Concentrations of 2 ng 6-MAM/mL can be detected using a 2-mL sample of urine. The method has been verified in urine samples of heroin abusers.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/17.1.48