Microscopic analysis of molar–incisor malformation

Objective Molar–incisor malformation (MIM) is a newly discovered type of dental anomaly that involves a characteristic root malformation of the permanent first molars. The aim of this study was to reveal the microstructure of MIM teeth in order to determine their origin. Study Design Four MIM teeth...

Full description

Saved in:
Bibliographic Details
Published in:Oral surgery, oral medicine, oral pathology and oral radiology oral medicine, oral pathology and oral radiology, 2015-05, Vol.119 (5), p.544-552
Main Authors: Lee, Hyo-Seol, DDS, MSD, Kim, Soo-Hyun, DDS, Kim, Seong-Oh, DDS, PhD, Choi, Byung-Jai, DDS, PhD, Cho, Sung-Won, DDS, PhD, Park, Wonse, DDS, PhD, Song, Je Seon, DDS, PhD
Format: Article
Language:English
Subjects:
Child
Dentistry
Female
Humans
Incisor - abnormalities
Incisor - ultrastructure
Microscopy, Electron, Scanning
Molar - abnormalities
Molar - surgery
Molar - ultrastructure
Surgery
Tooth Extraction
X-Ray Microtomography
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective Molar–incisor malformation (MIM) is a newly discovered type of dental anomaly that involves a characteristic root malformation of the permanent first molars. The aim of this study was to reveal the microstructure of MIM teeth in order to determine their origin. Study Design Four MIM teeth were extracted from a 9-year-old girl due to severe mobility. The detailed microstructure of the teeth was determined by examinations with micro-computed tomography (micro-CT), hematoxylin and eosin (H&E) staining, immunohistochemical staining, and scanning electron microscopy to reveal the detailed microstructure. Results Micro-CT and H&E staining revealed the pulpal floor comprising three layers: upper, middle, and lower. Amorphous hard tissues and hyperactive cells were observed in the middle layer of the pulpal floor, and the cells stained positively for dentin sialoprotein and osteocalcin, but not for collagen XII. Conclusion The results of the present study imply that MIM-affected molars probably result from inappropriate differentiation of the apical pulp and dental follicle.
ISSN:2212-4403
2212-4411
DOI:10.1016/j.oooo.2014.10.013