Expression of anti-inflammatory mediator lipoxin A4 and inflammation responsive transcriptive factors NF-kappa B in patients with preeclampsia

To evaluate the role of lipoxin A4 (LXA4), NF-kappaB p65 in preeclampsia. LXA4 in blood serum and the lipoxin A4 receptor (ALX-R), NF-kappaB p65 mRNA, protein expressions in placenta-specific tissues were obtained from patients with preeclampsia and normal pregnancy. Levels of lipoxin A4 in women wi...

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Bibliographic Details
Published in:Clinical and experimental obstetrics & gynecology 2014-01, Vol.41 (5), p.561-566
Main Authors: Huang, L L, Su, S, Awale, R, Zhang, X Y, Zhong, L L, Tang, H
Format: Article
Language:English
Subjects:
Enzyme-Linked Immunosorbent Assay
Female
Gene Expression Regulation
Humans
Immunohistochemistry
Inflammation - complications
Inflammation - genetics
Inflammation - metabolism
Lipoxins - biosynthesis
Lipoxins - genetics
Placenta - metabolism
Pre-Eclampsia - etiology
Pre-Eclampsia - genetics
Pre-Eclampsia - metabolism
Pregnancy
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
Transcription Factor RelA - biosynthesis
Transcription Factor RelA - genetics
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Summary:To evaluate the role of lipoxin A4 (LXA4), NF-kappaB p65 in preeclampsia. LXA4 in blood serum and the lipoxin A4 receptor (ALX-R), NF-kappaB p65 mRNA, protein expressions in placenta-specific tissues were obtained from patients with preeclampsia and normal pregnancy. Levels of lipoxin A4 in women with mild preeclampsia was significantly high (p < 0.05). There was no significant statistical difference between normal pregnancy and severe preeclampsia (p > 0.05). The mRNA expression of ALX-R was significantly low in women with preeclampsia than in control group (p < 0.01) and mRNA expression of NF- kappaB p65 was significantly high in preeclampsia (p < 0.01). The immunohistochemical staining of NF-kappaB p65 protein was stronger in severe preeclampsia group whereas staining of ALX-R in placental tissue was weaker than in control group (p < 0.01). ALX-R mRNA was negatively correlated with NF-kappaB (p < 0.0001), but there was no correlation between LXA4 and ALX-R mRNA. There was an excessive maternal inflammatory response in preeclampsia. LXA4, ALX-R, and NF-kappaB p65 may be involved in the disease process ofpreeclampsia.
ISSN:0390-6663
DOI:10.12891/ceog17902014