Molecular characterization of methicillin-resistant Staphylococcus aureus: characterization of major clones and emergence of epidemic clones of sequence type (ST) 36 and ST 121 in Tehran, Iran

Information about the molecular structure of MRSA strains provides significant insights into the epidemiology of this important pathogen. To investigate the molecular characteristics of MRSA isolates, MRSA isolates were subjected to molecular typing by means of spa typing, multilocus sequence typing...

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Bibliographic Details
Published in:FEMS microbiology letters 2015-04, Vol.362 (8), p.fnv043-fnv043
Main Authors: Ohadian Moghadam, Solmaz, Pourmand, Mohammad Reza, Mahmoudi, Mahmood, Sadighian, Hooman
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacterial Toxins - genetics
Chromosomes
Cluster analysis
DNA, Bacterial - genetics
Drug resistance
Epidemics
Epidemiology
Exotoxins - genetics
Humans
Iran
Leukocidin
Leukocidins - genetics
Methicillin
Methicillin Resistance - genetics
Methicillin-Resistant Staphylococcus aureus - classification
Methicillin-Resistant Staphylococcus aureus - drug effects
Methicillin-Resistant Staphylococcus aureus - genetics
Methicillin-Resistant Staphylococcus aureus - pathogenicity
Microbial Sensitivity Tests
Microbiology
Molecular Epidemiology
Molecular structure
Molecular Typing
Multilocus Sequence Typing
Multiplex Polymerase Chain Reaction
Penicillin-Binding Proteins
Prevalence
Spas
Staphylococcal Infections - epidemiology
Staphylococcal Infections - microbiology
Staphylococcus infections
Virulence Factors - genetics
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Summary:Information about the molecular structure of MRSA strains provides significant insights into the epidemiology of this important pathogen. To investigate the molecular characteristics of MRSA isolates, MRSA isolates were subjected to molecular typing by means of spa typing, multilocus sequence typing, Staphylococcal Cassette Chromosome mec (SCCmec) grouping and to phenotypic antimicrobial susceptibility testing by means of disk diffusion assay. Then the presence of pvl genes was evaluated. Cluster analysis by eBURSTv3 showed that MRSA isolates belonged to two major clonal complexes (CC); CC8 (ST239, ST585, ST2732, ST1294) and CC30 (ST30, ST36, ST1163) and four singletons. Subsequent analysis of MRSA isolates revealed that the most prevalent SCCmec type was type III (55.8%) followed by type IV (34.9%) and type II (2.3%). Totally 11 different spa types were discriminated among which types t037 and t030 were predominant. The prevalence of Panton-Valentine leukocidin (PVL)-positive MRSA strains was high (20%), which is a matter of great concern, because the PVL is frequently associated with severe and recurrent SSTIs. ST239-III- t037 represented the most predominant MRSA clone. The presence of sequence type (ST) 36 and ST 121 are being reported for the first time in Iran. Molecular characterization of MRSA.
ISSN:1574-6968
0378-1097
1574-6968
DOI:10.1093/femsle/fnv043