Exopolysaccharides from Cyanobacterium aponinum from the Blue Lagoon in Iceland increase IL-10 secretion by human dendritic cells and their ability to reduce the IL-17+ RORγt+ /IL-10+ FoxP3+ ratio in CD4+ T cells

• Published in: Immunology letters 2015-02, Vol.163 (2), p.157-162
• Main Authors: Gudmundsdottir, Asa B, Omarsdottir, Sesselja, Brynjolfsdottir, Asa, Paulsen, Berit S, Olafsdottir, Elin S, Freysdottir, Jona
• Format: Article
• Language: English
• Subjects: Allergy and Immunology; Allogeneic T cells; Blue Lagoon; CD4-Positive T-Lymphocytes - drug effects; CD4-Positive T-Lymphocytes - immunology; CD4-Positive T-Lymphocytes - metabolism; Cell Differentiation - immunology; Cells, Cultured; Coculture Techniques; Culture Media, Conditioned - metabolism; Culture Media, Conditioned - pharmacology; Cyanobacteria; Cyanobacteria - chemistry; Cyanobacterium aponinum; Dendritic cells; Dendritic Cells - drug effects; Dendritic Cells - immunology; Dendritic Cells - metabolism; Enzyme-Linked Immunosorbent Assay; Exopolysaccharides; Flow Cytometry; Forkhead Transcription Factors - immunology; Forkhead Transcription Factors - metabolism; Humans; Iceland; Immunologic Factors - pharmacology; Immunomodulating effects; Interleukin-10 - immunology; Interleukin-10 - secretion; Interleukin-17 - immunology; Interleukin-17 - metabolism; Lymphocyte Activation - immunology; Nuclear Receptor Subfamily 1, Group F, Member 3 - immunology; Nuclear Receptor Subfamily 1, Group F, Member 3 - metabolism; Polysaccharides, Bacterial - immunology; Polysaccharides, Bacterial - pharmacology; Water Microbiology
• ISSN: 0165-2478; 1879-0542
• DOI: 10.1016/j.imlet.2014.11.008

Highlights • Analysis of immunomodulation by exopolysaccharides from Cyanobacterium aponinum (EPS-Ca). • The EPS-Ca induced higher IL-10 levels in dendritic cells compared with control. • EPS-Ca-treated dendritic cells were co-cultured with allogeneic CD4+ T cells. • This led to lower ratio of IL-17+ RORγt+ /IL-10+ FoxP3+ CD4+ T cells compared with control.