Acute and Sub-acute Toxicity Profile of Carpolobia lutea Leaf Extract in Rats

• Published in: Journal of pharmacology & toxicology 2012, Vol.7 (3), p.140-149
• Main Authors: Nwidu, Lucky Legbosi, Samson Olu, Adesite, Nwafor, Paul Alozie
• Format: Article
• Language: English
• Subjects: Polygalaceae
• ISSN: 1816-496X; 2152-100X
• DOI: 10.3923/jpt.2012.140.149

Carpolobia lutea (CL) (Polygalaceae) leaf is proven pharmacologically as possessing anti-diarrheal properties. Sub-acute toxicological investigations were executed with the Crude Ethyl Acetate Extract (CEAE) and Ethyl Acetate Fraction (EAF) which earlier reports reveal potent pharmacological activities. The pathological changes were investigated in rodents over a period of 28 days adopting Good Laboratory Practice (GLP) conditions. Thirty adult male and female Wistar rats were randomized to four treatment groups of 6 rats/equal sex/group and were administered orally with three doses of CEAE, 192.5, 385.0, 770 and one dose of EAF 770.0 mg kg super(-1); while 10 mL kg super(-1) of 20% Tween 80 was used for the control test. Weekly relative body weights were evaluated. Blood samples were collected weekly for hematological examination. At autopsy, the major organs were carefully excised and weighed. Sections were collected for histological analysis. No visible sign of behavioral toxicity were observed during study. The acute toxicity study (LD sub(50)) shows that median lethal dose is 3850.0, 3240.4 and 1414.2 mg kg super(-1) for the ethanol fraction, crude ethyl acetate extract and ethyl acetate fraction, respectively. Body weight but not organ weight increased significantly (p