Hepatic microsomal mono-oxygenase activity and P450IA mRNA in North Sea dab Limanda limanda from contaminated sites

Mature pre-spawning female dab Limanda limanda were sampled during the Bremer-haven Workshop from stations in the southern North Sea along a transect heading approximately NW from the Elbe estuary (the 'German Bight Transect') and around an abandoned drilling site. Hepatic microsomal mono-...

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Bibliographic Details
Published in:Marine ecology. Progress series (Halstenbek) 1992-01, Vol.91 (1/3), p.65-69
Main Authors: Renton, K. W., Addison, R. F.
Format: Article
Language:English
Subjects:
Bays
BIOCHEMISTRY
Complementary DNA
Cytochromes
DNA
Drilling
Fish
Limanda limanda
Liver
Marine
Marine fishes
Messenger RNA
RNA
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Summary:Mature pre-spawning female dab Limanda limanda were sampled during the Bremer-haven Workshop from stations in the southern North Sea along a transect heading approximately NW from the Elbe estuary (the 'German Bight Transect') and around an abandoned drilling site. Hepatic microsomal mono-oxygenase activity as shown by ethoxyresorufin O-deethylase (EROD) and cyanoethoxycoumarln O-deethylase (CN-ECOD) declined with increasing distance offshore, but rose slightly at the outermost station on the Dogger Bank. Microsomal P4501A1 mRNA, detected with a new synthetic oligonucleotide probe, followed the same trend. Activities of EROD and CN-ECOD and concentrations of P4501A1 mRNA were well correlated with the major chlorobiphenyl residues measured in dab and with hydrocarbon concentrations in sediments at these stations. Dab sampled from the drilling site had hepatic EROD and CN-ECOD activity and P4501A1 mRNA contents similar to those in fish from the offshore German Bight stations.
ISSN:0171-8630
1616-1599
DOI:10.3354/meps091065