High Fermentative Production of L-Threonine from Acetate by a Brevibacterium flavum Stabilized Strain Transformed with a Recombinant Plasmid Carrying the Escherichia coli thr Operon

Decrease in L-threonine productivity caused not only by plasmid-free segregation but also by plasmid deletion was observed in a Brevibacterium flavum L-threonine producer when transformed with a recombinant plasmid carrying Escherichia coli thr operon. However, a recombinant strain, HT-16, was stabi...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1993-10, Vol.57 (10), p.1755-1756
Main Authors: Ishida, Masaaki, Sato, Katsuaki, Hashiguchi, Ken-ichi, Ito, Hisao, Enei, Hitoshi, Nakamori, Shigeru
Format: Article
Language:English
Subjects:
Acetates - metabolism
Bacteriology
Biological and medical sciences
Biotechnology
Brevibacterium - genetics
Brevibacterium - metabolism
Brevibacterium flavum
Culture Media
DNA, Recombinant
Escherichia coli
Escherichia coli - genetics
Fermentation - drug effects
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Genetic Vectors
Genetics
Methods. Procedures. Technologies
Microbiology
Operon
Plasmids
Stereoisomerism
Synthetic digonucleotides and genes. Sequencing
Threonine - biosynthesis
Transformation, Bacterial
Trimethoprim - pharmacology
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Summary:Decrease in L-threonine productivity caused not only by plasmid-free segregation but also by plasmid deletion was observed in a Brevibacterium flavum L-threonine producer when transformed with a recombinant plasmid carrying Escherichia coli thr operon. However, a recombinant strain, HT-16, was stabilized by the addition of trimethoprim (a selective marker on the vector) at concentration of 1000 /tg/ml, which was rather higher than the minimum inhibitory one, into the stock culture medium. Strain HT-16 produced 64.4 g/liter of L-threonine, 30% higher than that of the host strain, after 92 h of cultivation in a small jar fermentor using acetic acid as a carbon source without trimethoprim.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.57.1755