Chronic sleep deprivation alters the myosin heavy chain isoforms in the masseter muscle in rats

Abstract To investigate the changes in myosin heavy chain (MyHC) isoforms of rat masseter muscle fibres caused by chronic sleep deprivation and a possible link with the pathogenesis of disorders of the temporomandibular joint (TMJ). A total of 180 male rats were randomly divided into three groups (...

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Bibliographic Details
Published in:British journal of oral & maxillofacial surgery 2015-05, Vol.53 (5), p.430-435
Main Authors: Cao, Ruihua, Huang, Fei, Wang, Peihuan, Chen, Chen, Zhu, Guoxiong, Chen, Lei, Wu, Gaoyi
Format: Article
Language:English
Subjects:
Animals
Chronic sleep deprivation
Dentistry
Male
Masseter muscle
Masseter Muscle - chemistry
Muscle Fibers, Fast-Twitch - chemistry
Muscle Fibers, Slow-Twitch - chemistry
Myosin heavy chain
Myosin Heavy Chains - analysis
Protein Isoforms - analysis
qPCR
Random Allocation
Rats
Rats, Wistar
Sleep Deprivation - metabolism
Surgery
Temporomandibular joint disorder
Time Factors
Transcriptional change
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Summary:Abstract To investigate the changes in myosin heavy chain (MyHC) isoforms of rat masseter muscle fibres caused by chronic sleep deprivation and a possible link with the pathogenesis of disorders of the temporomandibular joint (TMJ). A total of 180 male rats were randomly divided into three groups ( n = 60 in each): cage controls, large platform controls, and chronic sleep deprivation group. Each group was further divided into three subgroups with different observation periods (7, 14, and 21 days). We investigated he expression of MyHC isoforms in masseter muscle fibres by real-time quantitative polymerase chain reaction (PCR), Western blotting, and immunohistochemical staining. In rats with chronic sleep deprivation there was increased MyHC-I expression in layers of both shallow and deep muscles at 7 and 21 days compared with the control groups, whereas sleep deprivation was associated with significantly decreased MyHC-II expression. At 21 days, there were no differences in MyHC-I or MyHC-II expression between the groups and there were no differences between the two control groups at any time point. These findings suggest that chronic sleep deprivation alters the expression of MyHC isoforms, which may contribute to the pathogenesis of disorders of the TMJ.
ISSN:0266-4356
1532-1940
DOI:10.1016/j.bjoms.2015.02.011