Microsatellite repeats in grapevine reveal DNA polymorphisms when analysed as sequence-tagged sites (STSs)

Microsatellite repeat sequences were investigated as sequenced-tagged site (STS) DNA markers to determine the potential for genetic analysis of the grapevine genome. The PCR-generated markers detect codominant alleles at a single locus or site in the genome. The marker type is very informative detec...

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Bibliographic Details
Published in:Theoretical and applied genetics 1993-09, Vol.86 (8), p.985-990
Main Authors: Thomas, M.R. (Commonwealth Scientific and Industrial Research Organisation, Adelaide, SA (Australia). Div. of Horticulture), Scott, N.S
Format: Article
Language:English
Subjects:
ADN
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
DNA polymorphisms
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Grapevine
MARCADORES GENETICOS
MARQUEUR GENETIQUE
Microsatellite DNA
POLIMORFISMO GENETICO
POLYMORPHISME GENETIQUE
Pteridophyta, spermatophyta
SECUENCIA NUCLEICA
SEQUENCE NUCLEIQUE
SSLP
STMS
STS
VARIEDADES
VARIETE
Vegetals
Vitis
VITIS VINIFERA
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Summary:Microsatellite repeat sequences were investigated as sequenced-tagged site (STS) DNA markers to determine the potential for genetic analysis of the grapevine genome. The PCR-generated markers detect codominant alleles at a single locus or site in the genome. The marker type is very informative detecting high heterozygosity (69%-88%) within individual grapevine cultivars and high genetic variation between cultivars, making it a useful marker type for plant genome mapping and genome typing. For five loci a screening of 26 V. vinifera cultivars found 13, 12, 8, 5, and 4 different length alleles respectively with some alleles more common than others. The genomic DNA sequences surrounding microsatellite sequences were conserved within the genus permitting STS primers to amplify STSs from other Vitis species. These Vitis species were found to have some unique alleles not present in V. vinifera.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00211051