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Microsatellite repeats in grapevine reveal DNA polymorphisms when analysed as sequence-tagged sites (STSs)
Microsatellite repeat sequences were investigated as sequenced-tagged site (STS) DNA markers to determine the potential for genetic analysis of the grapevine genome. The PCR-generated markers detect codominant alleles at a single locus or site in the genome. The marker type is very informative detec...
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Published in: | Theoretical and applied genetics 1993-09, Vol.86 (8), p.985-990 |
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container_title | Theoretical and applied genetics |
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creator | Thomas, M.R. (Commonwealth Scientific and Industrial Research Organisation, Adelaide, SA (Australia). Div. of Horticulture) Scott, N.S |
description | Microsatellite repeat sequences were investigated as sequenced-tagged site (STS) DNA markers to determine the potential for genetic analysis of the grapevine genome. The PCR-generated markers detect codominant alleles at a single locus or site in the genome. The marker type is very informative detecting high heterozygosity (69%-88%) within individual grapevine cultivars and high genetic variation between cultivars, making it a useful marker type for plant genome mapping and genome typing. For five loci a screening of 26 V. vinifera cultivars found 13, 12, 8, 5, and 4 different length alleles respectively with some alleles more common than others. The genomic DNA sequences surrounding microsatellite sequences were conserved within the genus permitting STS primers to amplify STSs from other Vitis species. These Vitis species were found to have some unique alleles not present in V. vinifera. |
doi_str_mv | 10.1007/BF00211051 |
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(Commonwealth Scientific and Industrial Research Organisation, Adelaide, SA (Australia). Div. of Horticulture)</creatorcontrib><creatorcontrib>Scott, N.S</creatorcontrib><title>Microsatellite repeats in grapevine reveal DNA polymorphisms when analysed as sequence-tagged sites (STSs)</title><title>Theoretical and applied genetics</title><addtitle>Theor Appl Genet</addtitle><description>Microsatellite repeat sequences were investigated as sequenced-tagged site (STS) DNA markers to determine the potential for genetic analysis of the grapevine genome. The PCR-generated markers detect codominant alleles at a single locus or site in the genome. The marker type is very informative detecting high heterozygosity (69%-88%) within individual grapevine cultivars and high genetic variation between cultivars, making it a useful marker type for plant genome mapping and genome typing. For five loci a screening of 26 V. vinifera cultivars found 13, 12, 8, 5, and 4 different length alleles respectively with some alleles more common than others. The genomic DNA sequences surrounding microsatellite sequences were conserved within the genus permitting STS primers to amplify STSs from other Vitis species. These Vitis species were found to have some unique alleles not present in V. vinifera.</description><subject>ADN</subject><subject>Biological and medical sciences</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>DNA polymorphisms</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Grapevine</subject><subject>MARCADORES GENETICOS</subject><subject>MARQUEUR GENETIQUE</subject><subject>Microsatellite DNA</subject><subject>POLIMORFISMO GENETICO</subject><subject>POLYMORPHISME GENETIQUE</subject><subject>Pteridophyta, spermatophyta</subject><subject>SECUENCIA NUCLEICA</subject><subject>SEQUENCE NUCLEIQUE</subject><subject>SSLP</subject><subject>STMS</subject><subject>STS</subject><subject>VARIEDADES</subject><subject>VARIETE</subject><subject>Vegetals</subject><subject>Vitis</subject><subject>VITIS VINIFERA</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNp90ctv1DAQB2ALgeiycOHIAfmAUEEKjB-pk2OfgFTg0HKOZp3xNlVe9WSL9r-vo92WGydLo29-8swI8VbBFwXgvp5cAGilIFfPxEJZozOtrX4uFgAWstzl-kC8Yr6FxHIwL8WBtqq0qXUhbn82Pg6ME7VtM5GMNBJOLJteriOOdN_0c_GesJVnv47lOLTbbojjTcMdy7831Evssd0y1RJZMt1tqPeUTbhepxKnTJaHV9dX_Om1eBGwZXqzf5fiz8X59en37PL3tx-nx5eZt-CmzBchBMqV0UTgwClb29w7VWBNWKQZqA62proOK3CrFZpc-9oFgtyTD-bILMXHXe4Yh_QbnqquYZ_mw56GDVfqyNliXsRSHP4fWltqV5bFTD_v6LwsjhSqMTYdxm2loJqPUP07QsLv97mbVUf1E33cegIf9gDZYxsi9r7hJ2cKZZ0pEnu3YwGHCtcxkbPz0pxoA6V5AP-Hl3E</recordid><startdate>19930901</startdate><enddate>19930901</enddate><creator>Thomas, M.R. 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Div. of Horticulture) ; Scott, N.S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-c8fffe5132ee070714d45c718adea8000edf4deddfb07bba352cd7fe05cecf363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>ADN</topic><topic>Biological and medical sciences</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>DNA polymorphisms</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Grapevine</topic><topic>MARCADORES GENETICOS</topic><topic>MARQUEUR GENETIQUE</topic><topic>Microsatellite DNA</topic><topic>POLIMORFISMO GENETICO</topic><topic>POLYMORPHISME GENETIQUE</topic><topic>Pteridophyta, spermatophyta</topic><topic>SECUENCIA NUCLEICA</topic><topic>SEQUENCE NUCLEIQUE</topic><topic>SSLP</topic><topic>STMS</topic><topic>STS</topic><topic>VARIEDADES</topic><topic>VARIETE</topic><topic>Vegetals</topic><topic>Vitis</topic><topic>VITIS VINIFERA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thomas, M.R. (Commonwealth Scientific and Industrial Research Organisation, Adelaide, SA (Australia). 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Div. of Horticulture)</au><au>Scott, N.S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microsatellite repeats in grapevine reveal DNA polymorphisms when analysed as sequence-tagged sites (STSs)</atitle><jtitle>Theoretical and applied genetics</jtitle><addtitle>Theor Appl Genet</addtitle><date>1993-09-01</date><risdate>1993</risdate><volume>86</volume><issue>8</issue><spage>985</spage><epage>990</epage><pages>985-990</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><coden>THAGA6</coden><abstract>Microsatellite repeat sequences were investigated as sequenced-tagged site (STS) DNA markers to determine the potential for genetic analysis of the grapevine genome. The PCR-generated markers detect codominant alleles at a single locus or site in the genome. The marker type is very informative detecting high heterozygosity (69%-88%) within individual grapevine cultivars and high genetic variation between cultivars, making it a useful marker type for plant genome mapping and genome typing. For five loci a screening of 26 V. vinifera cultivars found 13, 12, 8, 5, and 4 different length alleles respectively with some alleles more common than others. The genomic DNA sequences surrounding microsatellite sequences were conserved within the genus permitting STS primers to amplify STSs from other Vitis species. These Vitis species were found to have some unique alleles not present in V. vinifera.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>24194007</pmid><doi>10.1007/BF00211051</doi><tpages>6</tpages></addata></record> |
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ispartof | Theoretical and applied genetics, 1993-09, Vol.86 (8), p.985-990 |
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language | eng |
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source | Springer LINK Archives |
subjects | ADN Biological and medical sciences Classical genetics, quantitative genetics, hybrids DNA polymorphisms Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Grapevine MARCADORES GENETICOS MARQUEUR GENETIQUE Microsatellite DNA POLIMORFISMO GENETICO POLYMORPHISME GENETIQUE Pteridophyta, spermatophyta SECUENCIA NUCLEICA SEQUENCE NUCLEIQUE SSLP STMS STS VARIEDADES VARIETE Vegetals Vitis VITIS VINIFERA |
title | Microsatellite repeats in grapevine reveal DNA polymorphisms when analysed as sequence-tagged sites (STSs) |
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