Expression of glucocorticoid receptor isoforms and associations with serine/arginine-rich protein 30c and 40 in patients with systemic lupus erythematosus

To investigate the expression of glucocorticoid receptor (GR) isoforms in patients with systemic lupus erythematosus (SLE), confirm the main GR isoforms involving in glucocorticoids (GC) resistance, and explore the associations of GR isoforms with serine/arginine-rich protein (SRp) 30c and SRp40. Se...

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Bibliographic Details
Published in:Clinical and experimental rheumatology 2015-03, Vol.33 (2), p.225-233
Main Authors: Guan, Yan-Chun, Jiang, Lei, Ma, Liang-Liang, Sun, Xiang-Nan, Yu, Dan-Dan, Liu, Jing, Qu, Dong-Xia, Fang, Mei-Yun
Format: Article
Language:English
Subjects:
Adult
Biomarkers - blood
Case-Control Studies
Drug Resistance
Female
Glucocorticoids - therapeutic use
Humans
Leukocytes, Mononuclear - drug effects
Leukocytes, Mononuclear - metabolism
Lupus Erythematosus, Systemic - blood
Lupus Erythematosus, Systemic - diagnosis
Lupus Erythematosus, Systemic - drug therapy
Male
Middle Aged
Nuclear Proteins - blood
Nuclear Proteins - genetics
Predictive Value of Tests
Prednisone - therapeutic use
Protein Isoforms
Receptors, Glucocorticoid - agonists
Receptors, Glucocorticoid - blood
Receptors, Glucocorticoid - genetics
Risk Factors
RNA, Messenger - blood
RNA-Binding Proteins - blood
RNA-Binding Proteins - genetics
Serine-Arginine Splicing Factors
Severity of Illness Index
Time Factors
Treatment Outcome
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Summary:To investigate the expression of glucocorticoid receptor (GR) isoforms in patients with systemic lupus erythematosus (SLE), confirm the main GR isoforms involving in glucocorticoids (GC) resistance, and explore the associations of GR isoforms with serine/arginine-rich protein (SRp) 30c and SRp40. Seventy patients with SLE and thirty-eight age- and sex-matched controls were recruited. All patients received prednisone (0.5-1 mg/kg/d) as their routine therapy. According to the therapeutic effect, patients were divided into glucocorticoid-resistant (GCR) and glucocorticoid-sensitive (GCS) groups. Transcript levels of GRα, GRβ, GRγ, GR-P, SRp30c and SRp40 in peripheral blood mononuclear cells (PBMCs) were determined by real-time PCR. GRα and GRβ proteins were detected by western blotting. Trial registration number is ChiCTR-RCH-12002808. Four GR transcripts in SLE patients showed the following trend: GRα (51.85%) > GR-P (23.78%) > GRγ (13.08%) >GRβ (0.03%). GR-P transcript and ratio of GRα/GR-P in SLE patients were significantly higher than that in controls (p
ISSN:0392-856X