The reversible inhibition of pollen germination of Nicotiana tabacum L. : an entry into a transformation system

The hydrodynamics of mature pollen rehydration in Nicotiana tabacum was used to study reversible inhibition of pollen germination in vitro. Tobacco pollen was incubated for various times in media containing calcium, potassium and magnesium salts, boric acid, and exhibiting different osmotic pressure...

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Bibliographic Details
Published in:Plant cell reports 1994, Vol.13 (3-4), p.231-236
Main Authors: LOGUERCIO, L. L, TERMIGNONI, R. R, OZAKI, L. S
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Germination and dormancy
Nicotiana tabacum
Plant physiology and development
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Summary:The hydrodynamics of mature pollen rehydration in Nicotiana tabacum was used to study reversible inhibition of pollen germination in vitro. Tobacco pollen was incubated for various times in media containing calcium, potassium and magnesium salts, boric acid, and exhibiting different osmotic pressures as a function of sucrose concentration. Total inhibition of germination with complete viability preservation was achieved for 56 h by keeping the grains in medium with 80% sucrose, since typical percentages of germination and pollen tube lengths were recovered after this treatment. These effects were considered as consequences of natural osmoregulation of rehydration/germination in mature pollen. The possibility of applying these findings to the incubation of pollen with Agrobacterium tumefaciens to develop a pollen transformation method is discussed.
ISSN:0721-7714
1432-203X
DOI:10.1007/BF00239899