Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli

In this study, microbial production of succinic acid from macroalgae (i.e., Laminaria japonica) was investigated for the first time. The engineered Escherichia coli BS002 exhibited higher molar yield of succinic acid on mannitol (1.39±0.01mol/mol) than glucose (1.01±0.05mol/mol). After pretreatment...

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Bibliographic Details
Published in:Bioresource technology 2015-06, Vol.185, p.56-61
Main Authors: Bai, Bing, Zhou, Jie-min, Yang, Mao-hua, Liu, Yi-lan, Xu, Xiao-hui, Xing, Jian-min
Format: Article
Language:English
Subjects:
Escherichia coli - physiology
Genetic Enhancement - methods
Glucose - metabolism
Mannitol - metabolism
Metabolic Engineering - methods
Seaweed - chemistry
Succinic Acid - isolation & purification
Succinic Acid - metabolism
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Summary:In this study, microbial production of succinic acid from macroalgae (i.e., Laminaria japonica) was investigated for the first time. The engineered Escherichia coli BS002 exhibited higher molar yield of succinic acid on mannitol (1.39±0.01mol/mol) than glucose (1.01±0.05mol/mol). After pretreatment and enzymatic hydrolysis, L. japonica hydrolysate was mainly glucose (10.31±0.32g/L) and mannitol (10.12±0.17g/L), which was used as the substrate for succinic acid fermentation with the recombinant BS002. A final 17.44±0.54g/L succinic acid was obtained from the hydrolysate after 72h dual-phase fermentation. The yield was as high as 1.24±0.08mol/mol total sugar, which reached 73% of the maximum theoretical yield. The results demonstrate that macroalgae biomass represents a novelty and economical alternative feedstock for biochemicals production.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2015.02.081