Pro-oxidant, antioxidant and 7-ethoxyresorufin O-deethylase (EROD) activity responses in liver of Dab ( Limanda limanda) exposed to sediment contaminated with hydrocarbons and other chemicals

• Published in: Marine pollution bulletin 1993, Vol.26 (11), p.602-606
• Main Authors: Livingstone, David R., Lemaire, Philippe, Matthews, Anne, Peters, Laurence, Bucke, David, Law, Robin J.
• Format: Article
• Language: English
• Subjects: Agnatha. Pisces; Animal, plant and microbial ecology; Applied ecology; Biological and medical sciences; Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on vertebrates; Fundamental and applied biological sciences. Psychology; Limanda limanda; Marine
• ISSN: 0025-326X; 1879-3363
• DOI: 10.1016/0025-326X(93)90498-9

Dab were exposed to reference sediment (79 μg PAHs g −1 dry wt) or contaminated sediment (309 μg PAHs g −1 dry wt plus low PCBs) for up to 140 days, and the effects on liver cytochrome P4501A (EROD activity), antioxidant enzymes and oxidative damage (lipid peroxidation) examined. EROD activity in the contaminated condition increased ×80 from 25 to 80 days, decreasing to 22% of this activity after 140 days. EROD activity also increased in the reference condition, but only after 140 days. Superoxide dismutase (SOD) and catalase activities and lipid peroxidation (malonaldehyde equivalents) were higher in the contaminated than the reference condition after 80 days but not 140 days. No differences were seen in glutathione peroxidase activity. It is concluded that the changes in SOD, catalase and lipid peroxidation are indicative of contaminant-mediated oxidative stress.