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Response of foot-and-mouth disease virus C sub(3) Resende to immunological pressure exerted in vitro by antiviral polyclonal sera

The foot-and-mouth disease virus (FMDV) shows a remarkable antigenic variability. Like other RNA viruses, FMDV has a high mutation rate and it has been proposed that selection exerted by antibodies of the host could play a major role in its evolution. In this work, antiserum-resistant variants of FM...

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Bibliographic Details
Published in:Virus research 1995-01, Vol.36 (1), p.77-85
Main Authors: Schiappacassi, M, Rojas, E R, Carrillo, E, Campos, R
Format: Article
Language:English
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Summary:The foot-and-mouth disease virus (FMDV) shows a remarkable antigenic variability. Like other RNA viruses, FMDV has a high mutation rate and it has been proposed that selection exerted by antibodies of the host could play a major role in its evolution. In this work, antiserum-resistant variants of FMDV (Nr variants) were selected upon 25 serial passages of a cloned C sub(3) Resende strain on secondary monolayers of fetal bovine kidney (FBK-2) cells in the presence of subneutralizing levels of antiviral polyclonal sera (APS). After serial passage under immune selective pressure, the five Nr variant populations selected from five independent serial passages-their controls remaining unmodified-acquired the following characteristics: (i) increased resistance to neutralization by APS; (ii) five different antigenic specificities detected by enzyme-linked and neutralization assays using monoclonal antibodies; (iii) the same modification (residue 146, S to L) at the major antigenic site of VP1 (G-H loop, the 135-160 region); and (iv) specific changes for each Nr population outside the major antigenic site of VP1 at residues 46, 48 and 49 of the 40-60 region of VP1 (B-C loop). These results extend our previous work on selection of Nr variants using polyclonal sera, and add new information with regard to antigenic variation, mainly concerning the involvement of the 40-60 region of VP1 in the process of immune selection.
ISSN:0168-1702