MUTANTS OF Streptomyces cattleya DEFECTIVE IN THE SYNTHESIS OF A FACTOR REQUIRED FOR THIENAMYCIN PRODUCTION

Thienamycin non-producing mutants of Streptomydes cattleya were identified that displayed a cross-feeding relationship. A diffusible product from one of these mutants (RK-11) resulted in restoration of thienamycin production when fed to cultures of another mutant (RK-4). In vivo radiolabeling experi...

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Bibliographic Details
Published in:Journal of antibiotics 1994/09/25, Vol.47(9), pp.992-1000
Main Authors: BUCHAN, TIM, ROACH, CLAUDIA, RUBY, CAROLYN, TAYLOR, DEAN, PREISIG, CAROL, REEVES, CHRISTOPHER
Format: Article
Language:English
Subjects:
Antibiotics (antibacterial agents, antifungal agents)
Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents
Applied microbiology
Biological and medical sciences
Chromatography, High Pressure Liquid
Cystine - metabolism
Ethyl Methanesulfonate - pharmacology
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
Methionine - analogs & derivatives
Methionine - metabolism
Microbiology
Mutation
Streptomyces - drug effects
Streptomyces - genetics
Streptomyces - metabolism
Sulfur Radioisotopes
Temperature
Thienamycins - biosynthesis
Tritium
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Summary:Thienamycin non-producing mutants of Streptomydes cattleya were identified that displayed a cross-feeding relationship. A diffusible product from one of these mutants (RK-11) resulted in restoration of thienamycin production when fed to cultures of another mutant (RK-4). In vivo radiolabeling experiments were conducted to test whether the RK-11 mutant produced a late biosynthetic intermediate which contained a carbapenem ring and a cysteaminyl and/or a hydroxyethyl side chain. Both [35S]cystine and [methyl-3H]methionine were used to label the RK-11 product which was then fed to RK-4 cultures. None of the thienamycin subsequently produced by RK-4 converter cells was labeled, implying the lack of either side chain of the thienamycin molecule in the RK-11 product. Further stability studies suggested that the RK-11 product does not contain a carbapenem ring. Additional feeding experiments with RK-4 cells also ruled out the possibility that the RK-11 product is a co-factor necessary for thienamycin production. It is concluded that the RK-11 product may regulate expression of the thienamycin gene cluster.
ISSN:0021-8820
1881-1469
DOI:10.7164/antibiotics.47.992