Effect of Seminal Plasma on Post‐Thaw Quality and Functionality of Corriedale Ram Sperm Obtained by Electroejaculation and Artificial Vagina

We have already shown that seminal collection method affects seminal plasma composition and sperm quality in Corriedale rams. In this study, we evaluated the effect of seminal plasma collected by electroejaculation or artificial vagina on sperm resistance to cryodamage. Seminal plasma of five rams o...

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Bibliographic Details
Published in:Reproduction in domestic animals 2015-06, Vol.50 (3), p.386-392
Main Authors: Ledesma, A, Manes, J, Ríos, G, Aller, J, Cesari, A, Alberio, R, Hozbor, F
Format: Article
Language:English
Subjects:
acrosome
Animal reproduction
Animals
artificial vagina
Corriedale
cryopreservation
Cryopreservation - veterinary
Ejaculation - physiology
Electric Stimulation - methods
freezing
Infertility
Male
Plasma
plasma membrane
rams
sampling
Semen - physiology
Semen Preservation - veterinary
seminal plasma
Sheep - physiology
Sperm
thawing
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Summary:We have already shown that seminal collection method affects seminal plasma composition and sperm quality in Corriedale rams. In this study, we evaluated the effect of seminal plasma collected by electroejaculation or artificial vagina on sperm resistance to cryodamage. Seminal plasma of five rams of the Corriedale breed collected by artificial vagina or electroejaculation was added before freezing to sperm cells collected by the two methods, and post‐thaw quality parameters were evaluated. We found that seminal plasma has no effect on sperm resistance to cryodamage. However, we observed significantly higher percentages of sperm with intact and functional plasma membrane, intact acrosome and greater fertilizing potential after thawing in samples obtained by electroejaculation. This study demonstrates that sperm collected by electroejaculation are more resistant to damage caused by cryopreservation than those collected by artificial vagina.
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.12500