Liquid chromatography/tandem mass spectrometry method to determine boldenone in bovine liver tissues

• Published in: Journal of AOAC International 2014-09, Vol.97 (5), p.1476-1480
• Main Authors: Granja, Rodrigo H M M, Salerno, Alessandro G, de Lima, Andreia C, Montalvo, Cynthia, Reche, Karine V G, Giannotti, Fabio M, Wanschel, Amarylis C B A
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Boldenone; Cattle; Chromatography, Liquid - methods; Drug Residues - analysis; Health aspects; Liquid chromatography; Liver - chemistry; Mass spectrometry; Physiological aspects; Tandem Mass Spectrometry - methods; Testosterone - analogs & derivatives; Testosterone - analysis
• ISSN: 1060-3271; 1944-7922
• DOI: 10.5740/jaoacint.13-335

Boldenone, an androgenic steroid, is forbidden for use in meat production in most countries worldwide. Residues of this drug in food present a potential risk to consumers. A sensitive LC/MS/MS method for analysis of 17β-boldenone using boldenone-d3 as an internal standard was developed. An enzymatic hydrolysis and extraction using ethyl acetate, methanol, and hexane were performed in the sample preparation. Parameters such as decision limit (CCα), detection capability (CCβ), precision, recovery, and ruggedness were evaluated according to the Brazilian Regulation 24/2009 (equivalent to European Union Decision 2002/657/EC) and International Organization for Standardization/International Electrotechnical Commission 17025:2005. CCα and CCβ were determined to be 0.17 and 0.29 μg/kg, respectively. Average recoveries from bovine liver samples fortified with 1, 1.5, and 2 μg/kg were around 100%. A complete statistical analysis was performed on the results obtained, including an estimation of the method uncertainty. The method is considered robust after being subjected to day-to-day analytical variations and has been used as a standard method in Brazil to report boldenone levels in bovine liver.